Rabbit Polyclonal Follistatin antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FST.
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Publications
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- Drug testing and analysis 12:1636-16482020Detection of follistatin-based inhibitors of the TGF-β signaling pathways in serum/plasma by means of LC-HRMS/MS and Western blotting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatja Walpurgis,Tim Weigand,Andre Knoop,Andreas Thomas,Christian Reichel,Frank Dellanna,Mario ThevisPubMed 32959984
- Drug testing and analysis 11:1675-16972019Detection of black market follistatin 344.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristian Reichel,Günter Gmeiner,Mario ThevisPubMed 31758732
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human FST. Database link P19883
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1-0.2 µg/mL | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25-1 µg/mL | Notes This allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin using 100 μl/well. |
Associated Products
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Target data
Function
Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
Alternative names
Follistatin, FS, Activin-binding protein, FST
Recommended products
Rabbit Polyclonal Follistatin antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FST.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human FST. Database link P19883
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- Reconstitute with 1ml sterile PBS solution containing 0.1% BSA.
- Concentration
- Purification notes
This antibody was purified by affinity chromatography and then biotinylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Once frozen, avoid more than one freeze-thaw cycle.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Follistatin also known as FS or FST is a glycoprotein with an approximate mass of 32 kDa. This protein is part of the follistatin family and plays an important role in various physiological mechanisms. Follistatin synthesizes and secretes in different tissues including the liver gonads and skeletal muscles. It binds to and inhibits members of the transforming growth factor-beta (TGF-β) superfamily particularly activin. This binding prevents activin from interacting with its receptors.
- Biological function summary
Follistatin modulates multiple processes such as cell growth differentiation and reproductive physiology. It acts as a binding protein for activin neutralizing its activity. By preventing activin from engaging with its receptor complexes follistatin influences developmental and regenerative processes. Despite not being a part of a transmembrane complex its interaction with activin impacts extracellular interactions.
- Pathways
Follistatin plays a significant role in the regulation of the TGF-β signaling pathway and the muscle growth pathway. In the TGF-β pathway it counteracts the signaling of activin which influences cellular homeostasis and inflammatory responses. In the muscle growth pathway follistatin interferes with myostatin another member of the TGF-β family promoting muscle growth. This cross-talk with proteins such as myostatin demonstrates follistatin's influence in muscle regeneration and development.
- Associated diseases and disorders
Follistatin has clear associations with muscle-related and reproductive disorders. It is linked to muscle wasting diseases such as muscular dystrophy due to its inhibitory role on myostatin known to restrain muscle growth. Follistatin abnormalities can also contribute to conditions like polycystic ovary syndrome (PCOS) due to its regulation of reproductive processes. These ties display follistatin's vital interactions with proteins like activin and myostatin highlighting its importance in disease pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Biotin Anti-Follistatin antibody (ab47942)
All lanes: Anti-Follistatin antibody (Biotin) (ab47942) at 0.2 μg/mlLane 1: Marker
Unreduced Recombinant human Follistatin protein (Recombinant human Follistatin protein ab50163):
Lane 2: 250 ng
Lane 3: 125 ng
Lane 4: 62.5 ng
Lane 5: 31.25 ng
Lane 6: 15.625 ng
Lane 7: 7.8 ng
Lane 8: 3.9 ng
Lane 9: 1.95 ng
Lane 10: 0.975 ng
Lane 11: 0.4875 ng
Lane 12: 0.24 ng
All lanes: Western blot - Biotin Anti-Follistatin antibody (ab47942)
Predicted band size: 38 kDa
Sandwich ELISA - Biotin Anti-Follistatin antibody (ab47942)
To detect Human Follistatin by sandwich ELISA (using 100 mL/well) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Rabbit polyclonal to Follistatin (ab47941) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin.
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