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AB47942

Biotin Anti-Follistatin antibody

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(2 Publications)

Rabbit Polyclonal Follistatin antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FST.

View Alternative Names

Follistatin, FS, Activin-binding protein, FST

2 Images
Sandwich ELISA - Biotin Anti-Follistatin antibody (AB47942)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-Follistatin antibody (AB47942)

To detect Human Follistatin by sandwich ELISA (using 100 mL/well) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Rabbit polyclonal to Follistatin (ab47941) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin.

Western blot - Biotin Anti-Follistatin antibody (AB47942)
  • WB

Supplier Data

Western blot - Biotin Anti-Follistatin antibody (AB47942)

All lanes : Anti-Follistatin antibody (Biotin) (ab47942) at 0.2 μg/ml

Lane 1 : Marker

Unreduced Recombinant human Follistatin protein (ab50163) :

Lane 2 : 250 ng

Lane 3 : 125 ng

Lane 4 : 62.5 ng

Lane 5 : 31.25 ng

Lane 6 : 15.625 ng

Lane 7 : 7.8 ng

Lane 8 : 3.9 ng

Lane 9 : 1.95 ng

Lane 10 : 0.975 ng

Lane 11 : 0.4875 ng

Lane 12 : 0.24 ng

All lanes:

Western blot - Biotin Anti-Follistatin antibody (ab47942)

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB, sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FST.

P19883

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.25-1 µg/mL", "sELISA-species-notes": "<p>This allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin using 100 μl/well.</p>" } } }
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Product details

Once frozen, avoid more than one freeze-thaw cycle.
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute with 1ml sterile PBS solution containing 0.1% BSA.
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was purified by affinity chromatography and then biotinylated.
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Follistatin also known as FS or FST is a glycoprotein with an approximate mass of 32 kDa. This protein is part of the follistatin family and plays an important role in various physiological mechanisms. Follistatin synthesizes and secretes in different tissues including the liver gonads and skeletal muscles. It binds to and inhibits members of the transforming growth factor-beta (TGF-β) superfamily particularly activin. This binding prevents activin from interacting with its receptors.
Biological function summary

Follistatin modulates multiple processes such as cell growth differentiation and reproductive physiology. It acts as a binding protein for activin neutralizing its activity. By preventing activin from engaging with its receptor complexes follistatin influences developmental and regenerative processes. Despite not being a part of a transmembrane complex its interaction with activin impacts extracellular interactions.

Pathways

Follistatin plays a significant role in the regulation of the TGF-β signaling pathway and the muscle growth pathway. In the TGF-β pathway it counteracts the signaling of activin which influences cellular homeostasis and inflammatory responses. In the muscle growth pathway follistatin interferes with myostatin another member of the TGF-β family promoting muscle growth. This cross-talk with proteins such as myostatin demonstrates follistatin's influence in muscle regeneration and development.

Follistatin has clear associations with muscle-related and reproductive disorders. It is linked to muscle wasting diseases such as muscular dystrophy due to its inhibitory role on myostatin known to restrain muscle growth. Follistatin abnormalities can also contribute to conditions like polycystic ovary syndrome (PCOS) due to its regulation of reproductive processes. These ties display follistatin's vital interactions with proteins like activin and myostatin highlighting its importance in disease pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
See full target information FST
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Drug testing and analysis 12:1636-1648 PubMed32959984

2020

Detection of follistatin-based inhibitors of the TGF-β signaling pathways in serum/plasma by means of LC-HRMS/MS and Western blotting.

Applications

Unspecified application

Species

Unspecified reactive species

Katja Walpurgis,Tim Weigand,Andre Knoop,Andreas Thomas,Christian Reichel,Frank Dellanna,Mario Thevis

Drug testing and analysis 11:1675-1697 PubMed31758732

2019

Detection of black market follistatin 344.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Reichel,Günter Gmeiner,Mario Thevis
View all publications
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