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Rabbit Polyclonal G-CSF antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Csf3 aa 1 to C-terminus.

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Images

Western blot - Biotin Anti-G-CSF antibody (AB271267), expandable thumbnail
  • Sandwich ELISA - Biotin Anti-G-CSF antibody (AB271267), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Biotin

Storage buffer

Constituents: PBS, 0.1% BSA

Form

Lyophilized

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Mouse Csf3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09920

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBsELISA
Recombinant fragment - Mouse
Tested
Tested

Tested
Tested

Species

Recombinant fragment - Mouse

Dilution info

0.10000-0.20000 µg/mL

Notes

-

Tested
Tested

Species

Recombinant fragment - Mouse

Dilution info

-

Notes

-

Target data

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Alternative names

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Rabbit Polyclonal G-CSF antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Csf3 aa 1 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Conjugation

Biotin

Form

Lyophilized

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Mouse Csf3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09920
Purification technique

Affinity purification

Reconstitution

Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Biotin Anti-G-CSF antibody (ab271267), expandable thumbnail

    Western blot - Biotin Anti-G-CSF antibody (ab271267)

    Western blot analysis of varying levels of Recombinant murine G-CSF protein using ab271267 at 0.1 μg/ml.

    Used in conjunction with compatible secondary reagents the detection limit for recombinant G-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    All lanes: Western blot - Biotin Anti-G-CSF antibody (ab271267)

    Predicted band size: 22 kDa

  • Sandwich ELISA - Biotin Anti-G-CSF antibody (ab271267), expandable thumbnail

    Sandwich ELISA - Biotin Anti-G-CSF antibody (ab271267)

    Mouse mG-CSF was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271267. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mG-CSF protein.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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