Biotin Anti-GAD65 + GAD67 antibody [EPR19366]

3 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GAD65 + GAD67 antibody [EPR19366] (ab314398)

  IHC image of GAD65 + GAD67 staining in a section of formalin-fixed paraffin-embedded mouse normal cerebellum.
  Performed on a Leica BOND™ system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using Avidin/Biotin Blocking Kit ab64212. The section was then incubated with ab314398 at 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GAD65 + GAD67 antibody [EPR19366] (ab314398)

  IHC image of GAD65 + GAD67 staining in a section of formalin-fixed paraffin-embedded mouse normal pancreas.
  Performed on a Leica BOND™ system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using Avidin/Biotin Blocking Kit ab64212. The section was then incubated with ab314398 at 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GAD65 + GAD67 antibody [EPR19366] (ab314398)

  Negative staining of GAD65 + GAD67 in a section of formalin-fixed paraffin-embedded mouse normal skin.
  Performed on a Leica BOND™ system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using Avidin/Biotin Blocking Kit ab64212. The section was then incubated with ab314398 at 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.