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AB95679

Biotin Anti-Galectin 3 antibody [M3/38]

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(1 Publication)

Rat Monoclonal Galectin 3 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Mouse Lgals3.

View Alternative Names

Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, IgE-binding protein, L-34 galactoside-binding lectin, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, Lgals3

2 Images
Flow Cytometry - Biotin Anti-Galectin 3 antibody [M3/38] (AB95679)
  • Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-Galectin 3 antibody [M3/38] (AB95679)

ab95679 at 0.125μg dilution staining the monocyte population in normal human peripheral blood cells by Flow Cytometry followed by Streptavidin PE.

Flow Cytometry - Biotin Anti-Galectin 3 antibody [M3/38] (AB95679)
  • Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-Galectin 3 antibody [M3/38] (AB95679)

ab95679 at 0.125μg dilution staining Galectin 3 in C57BL/6 mouse bone marrow cells by Flow Cytometry (shown in blue) followed by Streptavidin PE.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

M3/38

Isotype

IgG2a

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Mouse Lgals3.

P16110

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.25 µg for 10^5 Cells", "FlowCyt-species-notes": "<p>ab95679 can be used at 0.25 μg/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10<sup>5</sup> to 10<sup>8</sup> cells/test.</p><p><a href='/en-us/products/primary-antibodies/biotin-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18445'>ab18445</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.25 µg for 10^5 Cells", "FlowCyt-species-notes": "<p>ab95679 can be used at 0.25 μg/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10<sup>5</sup> to 10<sup>8</sup> cells/test.</p><p><a href='/en-us/products/primary-antibodies/biotin-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18445'>ab18445</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.
Biological function summary

Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.

Pathways

Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.

Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration (PubMed : 15181153). Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus : acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes (By similarity). When secreted, interacts with NK cell-activating receptor NCR3/NKp30 acting as an inhibitory ligand which antagonizes NK cell attack (By similarity).
See full target information Lgals3

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunological methods 169:153-61 PubMed7510757

1994

Assessment of the anti-c-kit monoclonal antibody YB5.B8 in affinity magnetic enrichment of human lung mast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Y Okayama,T C Hunt,O Kassel,L K Ashman,M K Church
View all publications

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