JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314264

Biotin Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal GFAP antibody - conjugated to Biotin. Suitable for IHC-P, WB and reacts with Mouse, Human, Rat samples.

View Alternative Names

Glial fibrillary acidic protein, GFAP

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB314264)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB314264)

Negative staining of GFAP in a section of formalin-fixed paraffin-embedded mouse liver performed on a Leica BOND™ system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab314264 at 5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB314264)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB314264)

IHC image of GFAP staining in a section of formalin-fixed paraffin-embedded mouse brain performed on a Leica BOND™ system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab314264 at 5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Carrier free

    Anti-GFAP antibody [EPR1034Y] - BSA and Azide free

  • Unconjugated

    Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 660 APC

    APC Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • HRP

    HRP Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 578 PE

    PE Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1034Y

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Mouse, Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB233621

Mouse GFAP ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse GFAP ELISA Kit (AB233621)

  • 0
  • 0
Primary Antibodies

AB190565

Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB190565)

  • 5
  • 7
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB52642

Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB52642)

  • 4
  • 37
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
See full target information GFAP
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com