Biotin Anti-GFP antibody

• IHC-P

AbReview16271****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GFP antibody (AB6658)

ab6658 staining GFP in human melanoma cells recovered from nude mice by Immunocytochemistry/ immunoflurescence. Cells were fixed with formaldehyde, permeabilized with 0.25% Triton X-100 RT for 10min and blocking with commercially available blocking buffer was performed for 30 minutes at RT. Samples were incubated with primary antibody (1 : 50) for 18 hours at 4°C. An Alexa Fluor^®488-conjugated donkey polyclonal to goat IgG was used as secondary antibody at 1/100 dilution. Green color indicates GFP/Fibrolast cells, while red color indicates Ki67 positive cells, most of them are tumor cells (Abcam`s ab15580 was used for the detection).

This image is a courtesy of Hongwei Shao

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Biotin Anti-GFP antibody (AB6658)

Immunofluorescence Microscopy using ab6658. Tissue : Sf-1 : Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation : 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval : frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody : Goat anti- GFP was used at 1/500 dilution in free floating imunnohistochemistry to detect GFP. Secondary antibody : Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1 : 10,000 for 45 min at RT.Localization : Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining : eGFP as green fluorescent signal and sections were counterstained with DAPI.

• ICC/IF

AbReview25217****

Immunocytochemistry/ Immunofluorescence - Biotin Anti-GFP antibody (AB6658)

ab6658 staining GFP in rat brain cells infected with viruses containing GFP under a CMV promoter by Immunocytochemistry/ Immunofluorescence.Cells were fixed with formaldehyde, permeabilized using 0.2% Triton, blocked with 20% serum and then incubated with ab6658 at a 1 : 50 dilution for 20 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated rabbit polyclonal, used at a 1/200 dilution.

Image courtesy of Efrat Shema by Abreview.

• IHC - Wmt

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IHC - Wholemount - Biotin Anti-GFP antibody (AB6658)

Immunofluorescence Microscopy using ab6658. Tissue : Drosophila melanogaster late stage embryonic central nervous system. Fixation : 0.5% PFA. Antigen retrieval : not required. Primary antibody : Anti-GFP antibody at a 1/1,000 for 1 h at RT. Secondary antibody : AlexaFluor 488™ conjugated anti-Goat antibody at 1/300 for 45 min at RT. Panel A : shows a lateral view (ventral left). Panels B and C : shows ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). In all panels, anterior is up. Staining : tau-GFP cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) as green fluorescent signal.

• WB

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Western blot - Biotin Anti-GFP antibody (AB6658)

All lanes:

Western blot - Biotin Anti-GFP antibody (ab6658)

Predicted band size: 27 kDa

Observed band size: 28 kDa

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