Rabbit Polyclonal GM-CSF antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Mouse, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Csf2 aa 1 to C-terminus.
View Alternative Names
Csfgm, Csf2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF
- sELISA
Supplier Data
Sandwich ELISA - Biotin Anti-GM-CSF antibody (AB271265)
Mouse GM-CSF was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271265. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mGM-CSF protein.
- WB
Supplier Data
Western blot - Biotin Anti-GM-CSF antibody (AB271265)
Western blot analysis of varying levels of Recombinant murine GM-CSF protein using ab271265 at 0.1 μg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant GM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24
All lanes:
Western blot - Biotin Anti-GM-CSF antibody (ab271265)
Predicted band size: 16 kDa
false
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Reactivity data
Properties and storage information
Form
Reconstitution
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.
Pathways
GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.
Product protocols
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Target data
Product promise
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