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AB84259

Biotin Anti-GM-CSF antibody

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(1 Publication)

Rabbit Polyclonal GM-CSF antibody - conjugated to Biotin. Carrier free. Suitable for WB, sELISA and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CSF2.

View Alternative Names

GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

Yes

Reacts with

Human

Applications

WB, sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CSF2.

P04141

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Alternative Products

Primary Antibodies

AB9741

Anti-GM-CSF antibody

ELISA - Anti-GM-CSF antibody (AB9741)

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Primary Antibodies

AB9667

Anti-GM-CSF antibody

Sandwich ELISA - Anti-GM-CSF antibody (AB9667)

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Primary Antibodies

AB220888

Anti-GM-CSF antibody

Immunocytochemistry/ Immunofluorescence - Anti-GM-CSF antibody (AB220888)

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Primary Antibodies

AB221170

Anti-GM-CSF antibody

Immunocytochemistry/ Immunofluorescence - Anti-GM-CSF antibody (AB221170)

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Primary Antibodies

AB106790

HRP Anti-GM-CSF antibody [KT35]

Sandwich ELISA - HRP Anti-GM-CSF antibody [KT35] (AB106790)

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Primary Antibodies

AB106746

Anti-GM-CSF antibody [KT37]

Sandwich ELISA - Anti-GM-CSF antibody [KT37] (AB106746)

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Complementary Products

Assay Kits

AB174448

Human GM-CSF ELISA Kit (CSF2)

Recombinant|SimpleStep

ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

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Primary Antibodies

AB106025

HRP Anti-IL-7 antibody

Western blot - HRP Anti-IL-7 antibody (AB106025)

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Primary Antibodies

AB181053

Anti-G-CSF antibody [EPR3203(N)(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (AB181053)

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  • 1
Assay Kits

AB174448

Human GM-CSF ELISA Kit (CSF2)

Recombinant|SimpleStep

ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

  • 0
  • 0
Primary Antibodies

AB242990

Anti-EGF antibody [EPR19899-46] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-EGF antibody [EPR19899-46] - BSA and Azide free (Detector) (AB242990)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>The detection limit for recombinant human GM-CSF is 1.5 - 3.0 ng/lane under either reducing or non-reducing conditions.</p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human GM-CSF. Can be paired for Sandwich ELISA with <a href='/en-us/products/primary-antibodies/gm-csf-antibody-ab9667'>ab9667</a>. Can be used as detection antibody when paired with recommended pair.</p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification technique
Affinity purification Protein A
Purification notes
ab84259 is sterile filtered.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GM-CSF also known as Granulocyte-Macrophage Colony-Stimulating Factor is a glycoprotein with a mass of approximately 23 kDa. This protein plays a mechanical role in stimulating the differentiation and proliferation of hematopoietic progenitor cells into granulocytes and macrophages. Its expression occurs in various cell types including macrophages T cells endothelial cells and fibroblasts. GM-CSF interacts with its specific receptor known as CSF2 receptor to exert its effects.
Biological function summary

GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.

Pathways

GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.

GM-CSF has a well-established connection to diseases like rheumatoid arthritis and multiple sclerosis. In rheumatoid arthritis overproduction of GM-CSF is linked to chronic inflammation and joint damage. In multiple sclerosis GM-CSF might contribute to the pathological immune responses against the central nervous system. Antibodies targeting GM-CSF or its receptor represent a therapeutic strategy for these autoimmune diseases. By modulating GM-CSF activity therapeutic approaches aim to reduce inflammation and autoimmunity highlighting the protein's importance in disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
See full target information CSF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

ACS applied materials & interfaces 15:25300-25312 PubMed37204867

2023

Toehold-Mediated Shape Transition of Nucleic Acid Nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan Hartung,Nathan McCann,Erwin Doe,Hannah Hayth,Kheiria Benkato,M Brittany Johnson,Mathias Viard,Kirill A Afonin,Emil F Khisamutdinov
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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