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Mouse Monoclonal HBEGF/DTR antibody - conjugated to Biotin. Suitable for IP, WB, ICC/IF and reacts with Transfected cell line - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HBEGF.
Alternative names=Proheparin-binding EGF-like growth factor, HBEGF, DTS, HEGFL, DTR
- Isotype
IgG1
- Host species
Mouse
- Conjugation
Biotin
- Storage buffer
pH: 6 - 8.5
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Recombinant Fragment Protein within Human HBEGF. The exact immunogen used to generate this antibody is proprietary information. Database link: Q99075
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Predicted | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Tested | Tested | Tested |
Images
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info 0.20000-1.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Target data
Function
Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
Recommended products
Mouse Monoclonal HBEGF/DTR antibody - conjugated to Biotin. Suitable for IP, WB, ICC/IF and reacts with Transfected cell line - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HBEGF.
Alternative names=Proheparin-binding EGF-like growth factor, HBEGF, DTS, HEGFL, DTR
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
Biotin
- Storage buffer
pH: 6 - 8.5
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Recombinant Fragment Protein within Human HBEGF. The exact immunogen used to generate this antibody is proprietary information. Database link: Q99075
- Clone number
4G10
- Purification technique
Affinity purification Protein G
- Epitope
EGF domain
- Concentration
- Purification notes
Produced in serum-free medium and purified by combination of chromatography.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Biotin/IgG = 7.5
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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