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AB66793

Biotin Anti-HBEGF/DTR antibody [4G10]

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(1 Publication)

Mouse Monoclonal HBEGF/DTR antibody - conjugated to Biotin. Suitable for IP, WB, ICC/IF and reacts with Transfected cell line - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HBEGF.

View Alternative Names

DTR, DTS, HEGFL, HBEGF, Proheparin-binding EGF-like growth factor

3 Images
Immunocytochemistry/ Immunofluorescence - Biotin Anti-HBEGF/DTR antibody [4G10] (AB66793)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Biotin Anti-HBEGF/DTR antibody [4G10] (AB66793)

These images shows Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR expression vector (right hand image). Cells treated with ab66793, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.

Immunoprecipitation - Biotin Anti-HBEGF/DTR antibody [4G10] (AB66793)
  • IP

Unknown

Immunoprecipitation - Biotin Anti-HBEGF/DTR antibody [4G10] (AB66793)

All lanes : HBEGF/DTR antibody [4G10](biotin) - Azide free (ab66793) at 1 μg/ml. Lane 1 : Vero cell extract Lane 2 : Vero cell extract from cells carrrying human HBEGF/DTR expression vector. Lane 3 : Vero cell extract from cells carrying mouse HBEGF/DTR expression vector Predicted band size : 23 kDa Observed band size : 20-38 kDa Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands. This image shows Immunoprecipitation of HBEGF/DTR followed by Western blotting/SDS PAGE; the cell surface was biotinylated.

Lane 1:

Immunoprecipitation - Anti-HBEGF/DTR antibody [4G10] (<a href='/en-us/products/primary-antibodies/hbegf-dtr-antibody-4g10-ab66792'>ab66792</a>)

Lane 1:

Immunoprecipitation - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)

Predicted band size: 23 kDa

Observed band size: 20-38 kDa,41 kDa

false

Western blot - Biotin Anti-HBEGF/DTR antibody [4G10] (AB66793)
  • WB

Unknown

Western blot - Biotin Anti-HBEGF/DTR antibody [4G10] (AB66793)

Lanes 1 - 3:

Western blot - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793) at 1 µg/mL

Lanes 1 - 3:

Western blot - Anti-HBEGF/DTR antibody [4G10] (<a href='/en-us/products/primary-antibodies/hbegf-dtr-antibody-4g10-ab66792'>ab66792</a>) at 1 µg/mL

Lane 1:

Vero (African green monkey kidney epithelial cell) whole cell lysate

Lane 2:

Vero cell extract from cells carrying human HBEGF/DTR expression vector

Lane 3:

Vero cell extract from cells carrying mouse HBEGF/DTR expression vector

Predicted band size: 23 kDa

Observed band size: 20-28 kDa,41 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4G10

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IP

applications

Immunogen

Recombinant Fragment Protein within Human HBEGF. The exact immunogen used to generate this antibody is proprietary information.

Q99075

Epitope

EGF domain

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Transfected cell line - Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Biotin/IgG = 7.5
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Produced in serum-free medium and purified by combination of chromatography.
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HBEGF also known as Heparin-binding EGF-like growth factor or Diphtheria Toxin Receptor (DTR) is a growth factor that binds to EGF receptors with its mass approximately 20 kilodaltons. It is a member of the EGF family. Cells secrete HBEGF and it is expressed in many tissues including the heart kidney and lungs. Its expression often increases during proliferation and differentiation making it an important player in tissue growth and repair.
Biological function summary

HBEGF functions as a mitogen and chemotactic agent which means it stimulates cell division and attracts cells to migrate. As part of the EGF family HBEGF activates the EGFR (epidermal growth factor receptor) signaling cascade. This signaling is essential for processes such as wound healing and development. HBEGF also forms complexes with other ligands and receptors enhancing its biological activity in tissues.

Pathways

HBEGF plays a role in the MAPK/ERK signaling pathway and the PI3K/AKT pathway. Both pathways are significant for regulating cell growth survival and differentiation. HBEGF interacts with other proteins like Ras and PI3K in these pathways. By activating these pathways HBEGF influences cellular responses critical for normal physiological processes and recovery mechanisms in cells.

HBEGF has connections with cancer and cardiovascular diseases. Aberrant HBEGF signaling can promote oncogenic processes in cancer relating it to proteins such as HER2 which also engage in similar pathways. In cardiovascular diseases HBEGF overexpression can contribute to smooth muscle cell proliferation an important event in atherosclerosis linking it to other growth factors involved in vascular biology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
See full target information HBEGF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:219 PubMed40542386

2025

Hsa_circ_0059511 promote glioma cell proliferation and migration through hsa-miR-194-5p/HBEGF axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Jing,Huang-Cheng Shang-Guan,Jiawei Cai,Jinye Su,Wei Huang,Qiu He,Xiaoyong Chen,Dezhi Kang,Zanyi Wu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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