AB68520

Biotin Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody

Name: Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) Biotin antibody (ab68520) | Abcam
Brand: Abcam Limited
SKU: AB68520
Price: 505 USD
Availability: InStock

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal HBSAG antibody - conjugated to Biotin. Suitable for ELISA, WB, ICC/IF, IHC-Fr and reacts with Hepatitis B virus samples. Cited in 5 publications. Immunogen corresponding to Virus preparation containing Hepatitis B Virus Surface Antigen (Ad/Ay) protein.

View Alternative Names

Large envelope protein, L glycoprotein, L-HBsAg, Large S protein, Large surface protein, Major surface antigen, LHB, S

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Hepatitis B virus

Applications

ELISA, ICC/IF, IHC-Fr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Reacts with Hepatitis B Virus Surface Antigen in viral and infected tissue samples.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis B virus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Hepatitis B Virus Surface Antigen (HBsAg) also known as Hepatitis B surface antigen AD/AY serves as a significant marker for the Hepatitis B virus (HBV). This glycoprotein displays a molecular mass of approximately 24 kDa and consists of small medium and large protein forms. HBsAg is typically expressed in the infected liver cells and secreted into the bloodstream. This expression allows its use as a diagnostic marker for HBV infection through assays like HBsAg ELISA which detects the presence of the virus.

Biological function summary

The surface antigen plays an important role in the virus's attachment and entry into hepatocytes. HBsAg forms part of the viral envelope structure which facilitates the virus's ability to infect host cells. The presence of HBsAg is linked to an individual’s immune response specifically the production of hepatitis antibodies. Its interaction with the host’s immune system and antibodies like hep B antibodies are essential for understanding both viral pathogenesis and immunity to HBV infections.

Pathways

The Hepatitis B virus surface antigen plays critical parts in the infectious cycle and immune response pathways. One important pathway includes the endocytosis pathway where HBsAg is involved in the virus's entry into liver cells. The surface antigen also interacts with the human immune system influencing the expression of Hepatitis B antibodies. Connected proteins include those involved in the immune signaling where alterations affect the body's ability to mount an effective response against HBV.

The Hepatitis B Virus Surface Antigen is directly associated with Hepatitis B infection and its progression. Chronic HBV infection may lead to liver-related diseases such as cirrhosis and hepatocellular carcinoma. HBsAg presence serves as an indicator for diagnosing these liver diseases. The antigen's interaction with other viral proteins such as the core antigen and E antigen are important in the disease's progression and overall clinical outcomes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.. The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.

See full target information S

Additional targets

S,S

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39000126

2024

Single-Cell RNA Sequencing Reveals the Spatial Heterogeneity and Functional Alteration of Endothelial Cells in Chronic Hepatitis B Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jingqi Shi,Qingyu Li,Jian Li,Jianglin Zhou,Xiaochang Zhang,Shengqi Wang,Liang Guo

Molecular carcinogenesis 63:1938-1952 PubMed38934768

2024

C5AR1-induced TLR1/2 pathway activation drives proliferation and metastasis in anaplastic thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Liu,Yueyao Sun,Tongyao Geng,Haobo Wang,Zhenyu Wu,Lei Xu,Miao Zhang,Xupeng Niu,Chenxu Zhao,Jin Shang,Fangjian Shang

NPJ vaccines 8:183 PubMed38001122

2023

Conjugation of HIV-1 envelope to hepatitis B surface antigen alters vaccine responses in rhesus macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Danielle Nettere,Shakthi Unnithan,Nicole Rodgers,Junsuke Nohara,Paul Cray,Madison Berry,Caroline Jones,Lawrence Armand,Shuk Hang Li,Stella J Berendam,Genevieve G Fouda,Derek W Cain,Taylor N Spence,Joshua A Granek,Clemontina A Davenport,Robert J Edwards,Kevin Wiehe,Koen K A Van Rompay,M Anthony Moody,Sallie R Permar,Justin Pollara

Viruses 15: PubMed38140543

2023

Replication and Expression of the Consensus Genome of Hepatitis B Virus Genotype C from the Chinese Population.

Applications

Unspecified application

Species

Unspecified reactive species

Fenfang Liao,Junmou Xie,Rongsong Du,Wenbo Gao,Lanyin Lan,Min Wang,Xia Rong,Yongshui Fu,Hao Wang

Frontiers in cellular and infection microbiology 10:74 PubMed32175289

2020

E-cadherin Plays a Role in Hepatitis B Virus Entry Through Affecting Glycosylated Sodium-Taurocholate Cotransporting Polypeptide Distribution.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Hu,Feifei Zhang,Liang Duan,Bo Wang,Yuanyuan Ye,Pu Li,Dandan Li,Shengjun Yang,Lan Zhou,Weixian Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (5)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise