AB122972

Biotin Anti-Hepatitis C Virus antibody [NS5B-6]

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(4 Publications)

Mouse Monoclonal Hepatitis C Virus antibody - conjugated to Biotin. Suitable for ELISA, WB, ICC/IF, Flow Cyt (Intra) and reacts with Hepatitis C virus samples. Cited in 4 publications.

View Alternative Names

Genome polyprotein, HCV, Hepatitis C virus polyprotein

2 Images

Immunocytochemistry/ Immunofluorescence - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (AB122972)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (AB122972)

ab122972, staining Hepatitis C Virus NS5b protein in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by immunofluorescence.

Unknown

Western blot - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (AB122972)

NS5b protein is detected as a 58kDa band.

All lanes:

Western blot - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)

All lanes:

Lysate of chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA

Predicted band size: 327 kDa

Observed band size: 58 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NS5B-6

Isotype

IgG2b

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Hepatitis C virus

Applications

ELISA, ICC/IF, Flow Cyt (Intra), WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis C virus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/biotin-mouse-igg2b-kappa-monoclonal-mpc-11-isotype-control-ab18418'>ab18418</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody." } } }

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Product details

Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Purification notes

filter-sterilized

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepatitis C Virus (HCV) is an RNA virus that causes liver infection. It has a small and compact genome of approximately 9.6 kilobases. Structurally HCV contains a single-stranded RNA encased in a nucleocapsid. This virus produces structural proteins like Core E1 and E2 and non-structural proteins such as NS3 NS4A NS4B NS5A and NS5B. These non-structural proteins play key roles in viral replication and assembly. Expression of HCV predominantly occurs in liver hepatocytes where the virus hijacks the host cellular machinery to replicate.

Biological function summary

Hepatitis C virus engages with host cell pathways to maintain its lifecycle. HCV forms replication complexes within host cells that are critical for its genome replication. These complexes actively interact with lipid metabolism processes which is why the virus associates closely with lipid droplets within hepatocytes. Through this interaction HCV affects host lipid homeostasis a component necessary for viral assembly and release. Since it forms structures that modify the host cell ER membrane understanding the HCV interaction with host cells is fundamental for therapeutic targeting.

Pathways

HCV has significant interactions with the innate immune response and lipid metabolism pathways. The virus disrupts interferon signaling by degrading proteins like IRF3 and blocking STAT1 phosphorylation undermining host antiviral defenses. Additionally HCV interplays with the PI3K-Akt-mTOR pathway important for host cell survival leveraging proteins such as Akt for its benefit. NS5A protein is known to modulate these pathways therefore influencing both the infection state and treatment strategies.

Chronic infection with HCV can lead to severe conditions like liver cirrhosis and hepatocellular carcinoma. The virus promotes hepatic fibrosis through continuous inflammation and liver cell damage. Additionally individuals with hepatitis C are at increased risk for developing metabolic disorders such as insulin resistance. Viral proteins like Core and NS5A drive these adverse outcomes by interacting with cell processes altering insulin signaling pathways and contributing to oncogenic transformations. Understanding these interactions is essential for developing effective HCV antibodies and therapeutic agents.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

See full target information Hepatitis C Virus

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 20:2238-2254 PubMed38842055

2024

ATG10S promotes IFNL1 expression and autophagic degradation of multiple viral proteins mediated by IFNL1.

Applications

Unspecified application

Species

Unspecified reactive species

Miao-Qing Zhang,Jian-Rui Li,Lu Yang,Zong-Gen Peng,Shuo Wu,Jing-Pu Zhang

Autophagy 16:2167-2179 PubMed31996071

2020

A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Miao-Qing Zhang,Qiong Zhao,Jing-Pu Zhang

Frontiers in immunology 9:2176 PubMed30319633

2018

Differential Effects of Autophagy-Related 10 Protein on HCV Replication and Autophagy Flux Are Mediated by Its Cysteine and Cysteine.

Applications

Unspecified application

Species

Unspecified reactive species

Miao-Qing Zhang,Jian-Rui Li,Zong-Gen Peng,Jing-Pu Zhang

Virology 198:636-44 PubMed8291245

1994

Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3.

Applications

Unspecified application

Species

Unspecified reactive species

S Manabe,I Fuke,O Tanishita,C Kaji,Y Gomi,S Yoshida,C Mori,A Takamizawa,I Yosida,H Okayama

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Biotin Anti-Hepatitis C Virus antibody [NS5B-6]

Immunocytochemistry/ Immunofluorescence - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (AB122972)

Western blot - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (AB122972)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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