AB193185

Biotin Anti-Human Ig light chain antibody [RM129]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal IGKC antibody - conjugated to Biotin. Suitable for ELISA, sELISA and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human IGKC.

View Alternative Names

Immunoglobulin kappa constant, Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, Ig kappa chain C region ROY, Ig kappa chain C region TI, IGKC

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Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)

sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)

A titer Sandwich ELISA using ab193185 as the detection antibody. The plate, coated with the capture antibody anti-human IgG, was loaded with different amounts of human IgG. A serial dilution of ab193185 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.

sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)

Sandwich ELISA using ab195581 as the capture antibody (100 ng/well), and Biotin Anti-Human Ig light chain antibody ab193185 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

ELISA

Supplier Data

ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)

ELISA of human immunoglobulins shows ab193185 reacts to both kappa and lambda light chains of human immunoglobulins. No cross reactivity with mouse, rat, or goat immunoglobulin light chain. The plate was coated with 50ng/well of different immunglobulins. 100 ng/mL or 20 ng/mL was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM129

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

ELISA, sELISA

applications

Immunogen

Full Length Protein corresponding to Human IGKC. The exact immunogen used to generate this antibody is proprietary information.

P01834

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.02-0.25 µg/mL", "sELISA-species-notes": "May be paired with <a href='/en-us/products/primary-antibodies/human-igg-antibody-rm116-ab195574'>ab195574</a>." }, "Mouse": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "0.02-0.25 µg/mL", "sELISA-species-notes": "May be paired with <a href='/en-us/products/primary-antibodies/human-igg-antibody-rm116-ab195574'>ab195574</a>." }, "Rat": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "0.02-0.25 µg/mL", "sELISA-species-notes": "May be paired with <a href='/en-us/products/primary-antibodies/human-igg-antibody-rm116-ab195574'>ab195574</a>." }, "Goat": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "0.02-0.25 µg/mL", "sELISA-species-notes": "May be paired with <a href='/en-us/products/primary-antibodies/human-igg-antibody-rm116-ab195574'>ab195574</a>." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Protein A affinity purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The human immunoglobulin light chain often referred to as "Ig light chain" or "IgL" is a component of antibodies. It's an essential part of the adaptive immune system. The light chains combine with heavy chains to form the complete immunoglobulin molecule. It has approximately 25 kDa mass. The light chain can be kappa (κ) or lambda (λ) types depending on the sequence. Expression occurs mainly in B lymphocytes where it plays a critical role in antibody diversity and specificity.

Biological function summary

The light chain contributes to antigen recognition by forming part of the antigen-binding site on an antibody. It's not a standalone entity but part of a larger immunoglobulin complex. This allows antibodies to bind specifically to antigens facilitating the immune response. The light chain also participates in stabilizing the antibody structure and ensuring proper immune function.

Pathways

The Ig light chain functions in the context of the adaptive immune system particularly in the immunoglobulin gene rearrangement and affinity maturation pathways. It works closely with the heavy chain to achieve effective antigen recognition. Additionally it interacts indirectly with proteins like B-cell receptor (BCR) which is important in the B lymphocyte signaling pathway. These interactions allow for the antibody-mediated detection and response to pathogens.

Abnormal Ig light chain production correlates with multiple myeloma and light chain amyloidosis. In multiple myeloma malignant plasma cells overproduce light chains often leading to their deposition in tissues and subsequent organ damage. In light chain amyloidosis the excess light chains misfold and deposit as amyloid fibrils causing organ dysfunction. Associations between these conditions and other immunoglobulin proteins highlight the role of Ig light chains in pathological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGKC

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Allergy, asthma, and clinical immunology : officia 16:30 PubMed32351585

2020

Local eosinophils are associated with increased IgA subclass levels in the sinonasal mucosa of chronic rhinosinusitis with polyp patients.

Applications

Unspecified application

Species

Unspecified reactive species

Hossein Aazami,Farhad Seif,Babak Ghalehbaghi,Pegah Babaheidarian,Alireza Mohebbi,Aslan Ahmadi,Majid Khoshmirsafa,Sahand Ghalehbaghi,Babak Behnam,Kobra Zinat Entezami,Zahra Madjd,Reza Falak

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Human Ig light chain antibody [RM129]

Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)

Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)

ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise