AB53270

Biotin Anti-IgA antibody [IgA5-3B]

Name: Anti-IgA Biotin antibody [IgA5-3B] (ab53270) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB53270
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Mouse Monoclonal IgA antibody - conjugated to Biotin. Suitable for ELISA and reacts with Common marmoset, Rhesus monkey, Human, Dog, Cat samples. Cited in 2 publications.

View Alternative Names

Hepatocellular carcinoma-associated protein TB6, Ig alpha 1 chain C region, Ig alpha 2 chain C region, Immunoglobulin heavy constant alpha 1, Immunoglobulin heavy constant alpha 2, Immunoglobulin heavy constant alpha 2 A2m marker, Poly-Ig receptor, polymeric immunoglobulin receptor, polymeric immunoglobulin receptor Secretory component

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IgA5-3B

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Cat, Dog, Human, Rhesus monkey, Common marmoset

Applications

ELISA

applications

Specificity

ab53270 recognises IgA. It does not cross-react with IgG or IgM; it has been shown to detect secreted IgA in cat saliva and rhesus monkey jejunal washes

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Cat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Common marmoset": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Dog": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Rhesus monkey": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab53270 is prepared by affinity chromatography on Protein A from tissue culture supernatant.

Storage buffer

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Immunoglobulin A (IgA) also known as sIgA in its secretory form is an important component of the immune system. With a molecular weight of approximately 160 kDa IgA exists as a monomeric or dimeric structure. It is primarily expressed in mucosal areas such as the respiratory tract gastrointestinal tract and urogenital tract while also being present in secretions like saliva tears and breast milk. In its dimeric form IgA associates with a joining (J) chain and a secretory component which facilitates its transport across mucosal barriers.

Biological function summary

IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.

Pathways

IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.

Alterations or deficiencies in IgA can lead to increased susceptibility to infections and disorders like IgA nephropathy and celiac disease. In IgA nephropathy IgA deposits in the kidneys result in inflammation and damage to renal tissues. Celiac disease involves an immune reaction to gluten where IgA antibodies against tissue transglutaminase (tTG) contribute to intestinal damage. Both conditions illustrate the importance of IgA in maintaining immune balance and its connection with disease pathologies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information IgA

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational gastroenterology 14:e00639 PubMed37753949

2023

Serological Investigation of Persistent Villous Atrophy in Celiac Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Changlin Gong,Claudia Saborit,Xin Long,Ao Wang,Beishi Zheng,Howard Chung,Suzanne K Lewis,Suneeta Krishnareddy,Govind Bhagat,Peter H R Green,Xiao-Fei Kong

ACS pharmacology & translational science 4:1818-1834 PubMed34927013

2021

Comprehensive Characterization of the LSD1 Small Molecule Inhibitor Class in Oncology.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Sacilotto,Paola Dessanti,Michele M P Lufino,Alberto Ortega,Alejandra Rodríguez-Gimeno,Jordi Salas,Tamara Maes,Carlos Buesa,Cristina Mascaró,Robert Soliva

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-IgA antibody [IgA5-3B]

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise