AB222777

Biotin Anti-IgA antibody [RM220]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal IgA antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Hepatocellular carcinoma-associated protein TB6, Ig alpha 1 chain C region, Ig alpha 2 chain C region, Immunoglobulin heavy constant alpha 1, Immunoglobulin heavy constant alpha 2, Immunoglobulin heavy constant alpha 2 A2m marker, Poly-Ig receptor, polymeric immunoglobulin receptor, polymeric immunoglobulin receptor Secretory component

3 Images

ELISA - Biotin Anti-IgA antibody [RM220] (AB222777)

ELISA

Supplier Data

ELISA - Biotin Anti-IgA antibody [RM220] (AB222777)

A titer ELISA of mouse IgA. The plate was coated with different amount of mouse IgA. A serial dilution of ab222777 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

ELISA

Supplier Data

ELISA - Biotin Anti-IgA antibody [RM220] (AB222777)

ELISA of mouse immunoglobulins shows ab222777 reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. The plate was coated with 50 ng/well of different immunoglobulins. ab222777 at 200 ng/ml, 50 ng/ml or 10 ng/ml was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

Supplier Data

Western blot - Biotin Anti-IgA antibody [RM220] (AB222777)

ab222777 reacts with nonreduced IgA.

All lanes:

Western blot - Biotin Anti-IgA antibody [RM220] (ab222777) at 0.5 µg/mL

Lane 1:

Mouse IgA (non-reduced)

Lane 2:

Mouse IgA (reduced)

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM220

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Mouse

Applications

ELISA, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.005-2 g/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 g/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Immunoglobulin A (IgA) also known as sIgA in its secretory form is an important component of the immune system. With a molecular weight of approximately 160 kDa IgA exists as a monomeric or dimeric structure. It is primarily expressed in mucosal areas such as the respiratory tract gastrointestinal tract and urogenital tract while also being present in secretions like saliva tears and breast milk. In its dimeric form IgA associates with a joining (J) chain and a secretory component which facilitates its transport across mucosal barriers.

Biological function summary

IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.

Pathways

IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.

Alterations or deficiencies in IgA can lead to increased susceptibility to infections and disorders like IgA nephropathy and celiac disease. In IgA nephropathy IgA deposits in the kidneys result in inflammation and damage to renal tissues. Celiac disease involves an immune reaction to gluten where IgA antibodies against tissue transglutaminase (tTG) contribute to intestinal damage. Both conditions illustrate the importance of IgA in maintaining immune balance and its connection with disease pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information IgA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 20:20220913 PubMed40226364

2025

Stigmasterol alleviates endplate chondrocyte degeneration through inducing mitophagy by enhancing PINK1 mRNA acetylation via the ESR1/NAT10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Li,Xiaofeng Chen,Baoci Huang,Junjie He,Junxian Xie,Weijun Guo,Jinjun Liang,Jiajian Ruan,Jincheng Liu,Zhen Xiang,Lixin Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-IgA antibody [RM220]

ELISA - Biotin Anti-IgA antibody [RM220] (AB222777)

ELISA - Biotin Anti-IgA antibody [RM220] (AB222777)

Western blot - Biotin Anti-IgA antibody [RM220] (AB222777)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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