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Rabbit Recombinant Monoclonal IgD antibody - conjugated to Biotin. Suitable for IHC, ELISA, sELISA and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human Immunoglobulin heavy constant delta.

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Images

Immunohistochemistry - Biotin Anti-IgD antibody [RM123] (AB224182), expandable thumbnail
  • ELISA - Biotin Anti-IgD antibody [RM123] (AB224182), expandable thumbnail
  • ELISA - Biotin Anti-IgD antibody [RM123] (AB224182), expandable thumbnail
  • Sandwich ELISA - Biotin Anti-IgD antibody [RM123] (AB224182), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Biotin

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human Immunoglobulin heavy constant delta. Database link P01880

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHCELISAsELISA
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Use: 25 - 200 ng/well (for capture); 0.01 - 0.1 μg/ml (for detection).

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. Soluble IgD (sIgD) concentration in serum below those of IgG, IgA, and IgM but much higher than that of IgE. IgM and IgD molecules present on B cells have identical V regions and antigen-binding sites. After the antigen binds to the B-cell receptor, the secreted form sIgD is shut off. IgD is a potent inducer of TNF, IL1B, and IL1RN. IgD also induces release of IL6, IL10, and LIF from peripheral blood mononuclear cells. Monocytes seem to be the main producers of cytokines in vitro in the presence of IgD (PubMed:8774350, PubMed:10702483, PubMed:11282392).

Alternative names

Immunoglobulin heavy constant delta, Ig delta chain C region, Ig delta chain C region NIG-65, Ig delta chain C region WAH, IGHD

Recommended products

Rabbit Recombinant Monoclonal IgD antibody - conjugated to Biotin. Suitable for IHC, ELISA, sELISA and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human Immunoglobulin heavy constant delta.

Alternative names

Immunoglobulin heavy constant delta, Ig delta chain C region, Ig delta chain C region NIG-65, Ig delta chain C region WAH, IGHD

Key facts

Isotype

IgG

Conjugation

Biotin

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Native Full Length Protein corresponding to Human Immunoglobulin heavy constant delta. Database link P01880
Clone number

RM123

Purification technique

Affinity purification Protein A

Specificity

ab224182 reacts to human IgD. No cross reactivity with human IgG, IgM, IgA, or IgE.

Concentration
Loading...
Purification notes

ab224182 was purified from an animal origin–free culture supernatant .

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C, Store in the dark

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

IgD also known as Immunoglobulin D serves as a distinct antibody isotype expressed primarily on the surface of B cells. It is involved in the initiation of immune responses. IgD has a molecular weight of approximately 180 kDa. The full form of IgD is Immunoglobulin D. It is expressed mainly in the respiratory system the tonsils and lymphoid tissues playing a pivotal role in the early stages of immunity. Researchers often perform IgD tests to study its mechanical aspects and influence on immune cell functionality.

Biological function summary

IgD contributes to the activation and differentiation of B lymphocytes and may influence antibody affinity maturation. This immunoglobulin is not known to form part of a larger complex but acts in conjunction with other immunoglobulins like IgM to regulate immune responses. The presence of IgD on B cells suggests its involvement in signaling processes which help B cells transition from immature to fully functional states impacting how the immune system responds to pathogens.

Pathways

IgD plays roles in immune system signaling and pathogen recognition. It appears in pathways such as B cell receptor signaling and participates in complex interactions with other proteins such as IgM and CD79 which are essential for transmitting activation signals. These pathways are important enabling the immune system to identify and respond to antigens effectively offering a first line of defense against infections.

Associated diseases and disorders

IgD has associations with recurrent respiratory infections and IgD myeloma a rare form of plasma cell neoplasm. The malfunction or dysregulation of IgD can impact B cell development and lead to immune system disorders. In such cases IgD levels often measured through IgD tests may serve as important indicators for monitoring disease progression. Moreover elevated levels of IgD can coexist with proteins such as IgE particularly in allergy-related responses and play roles in certain immunodeficiency conditions.

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4 product images

  • Immunohistochemistry - Biotin Anti-IgD antibody [RM123] (ab224182), expandable thumbnail

    Immunohistochemistry - Biotin Anti-IgD antibody [RM123] (ab224182)

    Human lymphoid tissue stained for IgD with ab224182 (2 μg/ml) in immunohistochemical analysis.

  • ELISA - Biotin Anti-IgD antibody [RM123] (ab224182), expandable thumbnail

    ELISA - Biotin Anti-IgD antibody [RM123] (ab224182)

    A titre ELISA using ab224182. The plate was coated with different amounts of human IgD from plasma. A serial dilution of ab224182 was used as primary antibody. An alkaline phosphatase-conjugated Anti-Rabbit IgG was used as secondary antibody.

  • ELISA - Biotin Anti-IgD antibody [RM123] (ab224182), expandable thumbnail

    ELISA - Biotin Anti-IgD antibody [RM123] (ab224182)

    ELISA of human immunoglobulins shows that ab224182 reacts to IgD from human plasma and IgDκ from human melanoma. No cross reactivity with human IgG, IgM, IgA or IgE. The plate was coated with 50 ng/well of the different immunoglobulins. 200 ng/ml, 50 ng/ml or 10 ng/ml of ab224182 was used as the primary antibody. An alkaline phosphatase-conjugated Anti-Rabbit IgG was used as secondary antibody.

  • Sandwich ELISA - Biotin Anti-IgD antibody [RM123] (ab224182), expandable thumbnail

    Sandwich ELISA - Biotin Anti-IgD antibody [RM123] (ab224182)

    This data was developed using the same antibody clone Anti-IgD antibody [RM123] ab195581 (unbiotinylated).

    Sandwich ELISA using Anti-IgD antibody [RM123] ab195581 as the capture antibody (100 ng/well), and Biotin Anti-Human Ig light chain antibody Biotin Anti-Human Ig light chain antibody [RM129] ab193185 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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