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AB246315

Biotin Anti-IGFBP1 antibody

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(1 Publication)

Rabbit Polyclonal IGFBP1 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IGFBP1.

View Alternative Names

IBP1, IGFBP1, Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1, Placental protein 12, PP12

2 Images
Sandwich ELISA - Biotin Anti-IGFBP1 antibody (AB246315)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-IGFBP1 antibody (AB246315)

To detect human IGFBP1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab246315 is required. This biotinylated polyclonal antibody, in conjunction with a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human IGFBP1.

Western blot - Biotin Anti-IGFBP1 antibody (AB246315)
  • WB

Supplier Data

Western blot - Biotin Anti-IGFBP1 antibody (AB246315)

To detect human IGFBP1 by Western Blot analysis ab246315 can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IGFBP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Lane 1 : Marker.

Lanes 2-12 : 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng/lane respectively.

All lanes:

Western blot - Biotin Anti-IGFBP1 antibody (ab246315)

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

sELISA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IGFBP1.

P08833

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.25-1 µg/mL", "sELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Purification technique
Affinity purification
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including cell migration, proliferation, differentiation or apoptosis in a cell-type specific manner (PubMed : 11397844, PubMed : 15972819). Plays also a positive role in cell migration by interacting with integrin ITGA5 : ITGB1 through its RGD motif (PubMed : 7504269). Mechanistically, binding to integrins leads to activation of focal adhesion kinase/PTK2 and stimulation of the mitogen-activated protein kinase (MAPK) pathway (PubMed : 11397844). Regulates cardiomyocyte apoptosis by suppressing HIF-1alpha/HIF1A ubiquitination and subsequent degradation (By similarity).
See full target information IGFBP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109862 PubMed38784022

2024

MDM2/MDMX inhibition by Sulanemadlin synergizes with anti-Programmed Death 1 immunotherapy in wild-type p53 tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Katrine Ingelshed,Marit M Melssen,Pavitra Kannan,Arun Chandramohan,Anthony W Partridge,Long Jiang,Fredrik Wermeling,David P Lane,Marika Nestor,Diana Spiegelberg
View all publications
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Product promise

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