Rabbit Polyclonal IGFBP1 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IGFBP1.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human IGFBP1. Database link P08833
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Human | Predicted | Predicted |
| Recombinant full length protein - Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.1-0.2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.25-1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including cell migration, proliferation, differentiation or apoptosis in a cell-type specific manner (PubMed:11397844, PubMed:15972819). Plays also a positive role in cell migration by interacting with integrin ITGA5:ITGB1 through its RGD motif (PubMed:7504269). Mechanistically, binding to integrins leads to activation of focal adhesion kinase/PTK2 and stimulation of the mitogen-activated protein kinase (MAPK) pathway (PubMed:11397844). Regulates cardiomyocyte apoptosis by suppressing HIF-1alpha/HIF1A ubiquitination and subsequent degradation (By similarity).
Alternative names
IBP1, IGFBP1, Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1, Placental protein 12, PP12
Recommended products
Rabbit Polyclonal IGFBP1 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IGFBP1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human IGFBP1. Database link P08833
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Biotin Anti-IGFBP1 antibody (ab246315)
To detect human IGFBP1 by Western Blot analysis ab246315 can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IGFBP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker.
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng/lane respectively.
All lanes: Western blot - Biotin Anti-IGFBP1 antibody (ab246315)
Predicted band size: 28 kDa
Sandwich ELISA - Biotin Anti-IGFBP1 antibody (ab246315)
To detect human IGFBP1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab246315 is required. This biotinylated polyclonal antibody, in conjunction with a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human IGFBP1.
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Product protocols
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