AB224181

Biotin Anti-IgG3 antibody [RM119]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal IGHG3 antibody - conjugated to Biotin. Suitable for IHC, ELISA, sELISA and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human IGHG3.

View Alternative Names

Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3

5 Images

ELISA - Biotin Anti-IgG3 antibody [RM119] (AB224181)

ELISA

Supplier Data

ELISA - Biotin Anti-IgG3 antibody [RM119] (AB224181)

A titer ELISA using ab224181. The plate was coated with different amounts of human IgG3. A serial dilution of ab224181 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-IgG3 antibody [RM119] (AB224181)

Sandwich ELISA using ab224181 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by a HRP conjugated streptavidin.

ELISA

Supplier Data

ELISA - Biotin Anti-IgG3 antibody [RM119] (AB224181)

ELISA of human immunoglobulins shows ab224181 only reacted to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab224181 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-IgG3 antibody [RM119] (AB224181)

Sandwich ELISA using ab224181 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.

Supplier Data

Immunohistochemistry - Biotin Anti-IgG3 antibody [RM119] (AB224181)

Human lymphoid tissue stained for human IgG3 using ab224181 at 5 μg/ml in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM119

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

ELISA, IHC, sELISA

applications

Immunogen

Full Length Protein corresponding to Human IGHG3. The exact immunogen used to generate this antibody is proprietary information.

P01860

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHC-species-checked": "testedAndGuaranteed", "IHC-species-dilution-info": "2-5 µg/mL", "IHC-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "Use: 50 ng/well - 200 ng/well (for Capture); 0.05 μg/mL - 0.2 μg/mL (for Detection)." }, "Mouse": { "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "2-5 g/mL", "IHC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "Use: 50 ng/well - 200 ng/well (for Capture); 0.05 μg/mL - 0.2 μg/mL (for Detection)." }, "Rat": { "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "2-5 g/mL", "IHC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "Use: 50 ng/well - 200 ng/well (for Capture); 0.05 μg/mL - 0.2 μg/mL (for Detection)." }, "Goat": { "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "2-5 g/mL", "IHC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "Use: 50 ng/well - 200 ng/well (for Capture); 0.05 μg/mL - 0.2 μg/mL (for Detection)." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab224181 was purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IgG3 also known as gamma rat IgG3 and 1gG3 is an immunoglobulin G subclass involved in immune response. It has a molecular mass of approximately 150 kDa. This antibody is expressed in B cells and circulates throughout the bloodstream. It plays a mechanical role in immune defense by recognizing and binding to antigens assisting in pathogen neutralization and immune activation.

Biological function summary

IgG3 is part of a complex network within the immune system. It binds to native proteins on the surface of pathogens enhancing their recognition by phagocytes. IgG3 function includes efficient complement activation which is critical for the opsonization and elimination of antigens. Mouse IgG3 is often used in research to study similar immune functions in animal models.

Pathways

IgG3 is integral in the complement cascade and Fc receptor signaling pathways. It interacts with proteins such as C1q and FcγRIII which mediate antibody-dependent cellular cytotoxicity and phagocytosis. These pathways illustrate how IgG3 links innate and adaptive immune responses making it essential for maintaining immune homeostasis.

IgG3 has associations with autoimmune conditions like rheumatoid arthritis and certain infectious diseases. Abnormal IgG3 function can contribute to disease progression or resistance. It connects to other proteins like rheumatoid factor and other IgG subclasses through these conditions influencing the pathophysiology and potential therapeutic responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGHG3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2019:1970818 PubMed31093311

2019

CIAPIN1 Targeted NHE1 and ERK1/2 to Suppress NSCLC Cells' Metastasis and Predicted Good Prognosis in NSCLC Patients Receiving Pulmonectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Ying Zhou,Li Ma,Shannan Cao,Wei Gao,Qingqing Xiong,Kaiyuan Wang,Lili Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-IgG3 antibody [RM119]

ELISA - Biotin Anti-IgG3 antibody [RM119] (AB224181)

Sandwich ELISA - Biotin Anti-IgG3 antibody [RM119] (AB224181)

ELISA - Biotin Anti-IgG3 antibody [RM119] (AB224181)

Sandwich ELISA - Biotin Anti-IgG3 antibody [RM119] (AB224181)

Immunohistochemistry - Biotin Anti-IgG3 antibody [RM119] (AB224181)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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