Rabbit Polyclonal IL-1 alpha antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL1A aa 100 to C-terminus.
IgG
Rabbit
Biotin
Constituents: PBS, 0.1% BSA
Lyophilized
Polyclonal
WB | sELISA | |
---|---|---|
Human | Predicted | Predicted |
Recombinant fragment - Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info 0.10000-0.20000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, IL1A, IL1F1
Rabbit Polyclonal IL-1 alpha antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL1A aa 100 to C-terminus.
Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, IL1A, IL1F1
IgG
Rabbit
Biotin
Constituents: PBS, 0.1% BSA
Lyophilized
Polyclonal
Affinity purification
Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
To detect hIL-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human IL-1 alpha, respectively.
Non-reducing conditions.
All lanes: Western blot - Biotin Anti-IL-1 alpha antibody (ab271226)
Predicted band size: 31 kDa
Human hIL-1α was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271226. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-1α protein.
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