Rabbit Polyclonal IL-10 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Il10 aa 1 to C-terminus.
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Publications
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- Frontiers in cellular and infection microbiology 12:8511972022E3 Ligase FBXW7 Facilitates Immune Evasion by Modulating TNF-α Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingrui Song,Jin Chao,Xiaohong Hu,Xin Wen,Cairong Ding,Dan Li,Ding Zhang,Shanshan Han,Xiang Yu,Bo Yan,Zhu Jin,Yinhong Song,Jacqueline Gonzales,Laura E Via,Lu Zhang,Decheng WangPubMed 35651754
- International journal of molecular medicine 46:1551-15612020Extracellular vesicles derived from human placental mesenchymal stem cells alleviate experimental colitis in mice by inhibiting inflammation and oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiyun Duan et. al.PubMed 32945344
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Il10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P18893
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Mouse | Expected | Tested |
| Recombinant fragment - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 0.10000-0.20000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed:28473584).
Alternative names
Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF
Recommended products
Rabbit Polyclonal IL-10 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Il10 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Il10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P18893
- Purification technique
- Affinity purification
- Reconstitution
- Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Biotin Anti-IL-10 antibody (ab271261)
Western blot analysis of varying levels of Recombinant human IL-10 protein using ab271261 at 0.1 μg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant IL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24All lanes: Western blot - Biotin Anti-IL-10 antibody (ab271261)
Performed under non-reducing conditions.
Predicted band size: 20 kDa
Sandwich ELISA - Biotin Anti-IL-10 antibody (ab271261)
Mouse mIL-10 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271261. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least0.2-0.4 ng/ wellof recombinant mIL-10 protein.
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