Rabbit Polyclonal IL-15 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL15.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human IL15. Database link P40933
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Recombinant full length protein - Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.1-0.2 µg/mL | Notes Detection limit for recombinant Human protein: 1.5 - 3.0 ng/lane, under reducing or non-reducing conditions. |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.25-1 µg/mL | Notes Detection limit for recombinant Human protein: 0.2 - 0.4 ng/well, using 100 μl/well antibody solution. |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed:15123770). Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed:8178155, PubMed:9326248). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (PubMed:9326248). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits (PubMed:10233906, PubMed:23104097, PubMed:8026467). Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5 (PubMed:7568001). In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (By similarity).
Alternative names
Interleukin-15, IL-15, IL15
Recommended products
Rabbit Polyclonal IL-15 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL15.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human IL15. Database link P40933
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- Centrifuge vial prior to opening. Reconstitute in 50ul sterile PBS containing 0.1% BSA
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Biotin Anti-IL-15 antibody (ab83157)
To detect human IL-15 by Western Blot analysis ab83157 can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is reducing conditions; Human recombinant IL-15 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant IL-15 protein.
All lanes: Western blot - Biotin Anti-IL-15 antibody (ab83157)
Predicted band size: 18 kDa
Sandwich ELISA - Biotin Anti-IL-15 antibody (ab83157)
To detect human IL-15 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83157 is required. This biotinylated polyclonal antibody, in conjunction with anti-IL-15 (ab9581) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human IL-15.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com