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AB271230

Biotin Anti-IL-2 Receptor alpha antibody

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(1 Publication)

Rabbit Polyclonal IL2RA antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IL2RA aa 1-250.

View Alternative Names

CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA

2 Images
Sandwich ELISA - Biotin Anti-IL-2 Receptor alpha antibody (AB271230)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-IL-2 Receptor alpha antibody (AB271230)

Human sIL-2 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271230. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant Human sIL-2 Receptor α protein.

Western blot - Biotin Anti-IL-2 Receptor alpha antibody (AB271230)
  • WB

Supplier Data

Western blot - Biotin Anti-IL-2 Receptor alpha antibody (AB271230)

Western blot analysis of varying levels of Recombinant human IL-2 Receptor α protein using ab271230 at 0.2 μg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant IL-2 Receptor α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24

All lanes:

Western blot - Biotin Anti-IL-2 Receptor alpha antibody (ab271230)

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

WB, sELISA

applications

Immunogen

Recombinant Fragment Protein within Human IL2RA aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P01589

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in PBS, 0.1% BSA
Purification technique
Affinity purification
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
See full target information IL2RA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The journal of obstetrics and gynaecology research 49:2417-2426 PubMed37464974

2023

Human Wharton's jelly-derived mesenchymal stem cells prevent pregnancy loss in a rat by JAK/STAT-mediated immunomodulation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying Ding,Rongrong Wu,Beibei Jin,Chunyu Zhu,Yuquan Zhang,Xiaoqing Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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