Rabbit Polyclonal IL-4 antibody - conjugated to Biotin. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL4.
IgG
Rabbit
Biotin
Constituents: PBS
Lyophilized
Polyclonal
WB | |
---|---|
Recombinant full length protein - Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.10000-0.20000 µg/mL | Notes The detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed:1993171, PubMed:3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed:1993171). Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed:2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells (PubMed:10219247, PubMed:11526337, PubMed:18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed:7721895).
Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4
Rabbit Polyclonal IL-4 antibody - conjugated to Biotin. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL4.
IgG
Rabbit
Biotin
Constituents: PBS
Lyophilized
Polyclonal
Yes
Affinity purification Protein A
Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
ab84242 was purified by affinity chromatography and then biotinylated and filter sterilised.
Blue Ice
1-2 weeks
+4°C
-20°C
Avoid freeze / thaw cycle
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Full details and terms and conditions can be found here:
Terms & Conditions.
To detect human IL-4 by Western Blot analysis ab84242 can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Human recombinant IL-4 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant IL-4 protein.
All lanes: Western blot - Biotin Anti-IL-4 antibody (ab84242)
Predicted band size: 17 kDa
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