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Rabbit Polyclonal IL-8 antibody - conjugated to Biotin. Carrier free. Suitable for WB, sELISA and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CXCL8.

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Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Biotin
Storage buffer

Constituents: PBS

Form
Lyophilized
Clonality
Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human CXCL8. Database link P10145

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBsELISA
Human
Expected
Expected

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

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Target data

Function

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:18692776, PubMed:7636208). Also plays an important role in neutrophil activation (PubMed:2145175, PubMed:9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed:1840701, PubMed:1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed:11971003, PubMed:8662698).

Alternative names

Recommended products

Rabbit Polyclonal IL-8 antibody - conjugated to Biotin. Carrier free. Suitable for WB, sELISA and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CXCL8.

Key facts

Isotype
IgG
Conjugation
Biotin
Form
Lyophilized
Clonality
Polyclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human CXCL8. Database link P10145
Carrier free
Yes
Purification technique
Affinity purification Protein A
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Concentration
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Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Notes

A sterile filtered antibody solution was lyophilized

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IL-8 also known as CXCL8 is a cytokine with a molecular weight of approximately 8 kDa. It belongs to the CXC chemokine family and plays a role in the recruitment of neutrophils to sites of inflammation. Cells like monocytes macrophages and endothelial cells express IL-8 after activation by pro-inflammatory signals. The protein exhibits high expression in cells associated with the immune response and inflammation serving as a signaling molecule between these cell types.

Biological function summary

IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.

Pathways

IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.

Associated diseases and disorders

IL-8 shows a strong association with conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). It acts as a biomarker for these diseases often correlating with disease severity and inflammation levels. In rheumatoid arthritis IL-8 contributes to joint inflammation and degradation while in COPD it enhances airway inflammation and tissue damage. The cytokine's involvement in these diseases connects it with other inflammatory mediators such as MMPs and leukotrienes which collectively exacerbate disease pathology.

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