Rabbit Polyclonal IP10 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Rat, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Rat Cxcl10 aa 1 to C-terminus.
Images
Publications
- Molecular and cellular biochemistry 476:1751-17632021The circular RNA circSlc7a11 promotes bone cancer pain pathogenesis in rats by modulating LLC-WRC 256 cell proliferation and apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan-Wen Chen,Xiao-Xia Zhang,Zhu-Ding Peng,Zu-Min Xing,Yi-Wen Zhang,Ya-Lan LiPubMed 33433832
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
Lyophilized
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Cxcl10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P48973
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Rat | Expected | Tested |
| Recombinant full length protein - Rat | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Rat | Dilution info 0.10000-0.20000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Rat | Dilution info - | Notes - |
Target data
Function
Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (By similarity). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (By similarity). Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity) (PubMed:30257241, PubMed:30448292).
Alternative names
C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Interferon-inducible protein 10, Protein Mob-1, Small-inducible cytokine B10, Gamma-IP10, IP-10, Cxcl10, Inp10, Mob1, Scyb10
Recommended products
Rabbit Polyclonal IP10 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Rat, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Rat Cxcl10 aa 1 to C-terminus.
Alternative names
C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Interferon-inducible protein 10, Protein Mob-1, Small-inducible cytokine B10, Gamma-IP10, IP-10, Cxcl10, Inp10, Mob1, Scyb10
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Cxcl10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P48973
- Purification technique
Affinity purification
- Reconstitution
Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
IP10 also known as CXCL10 is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of approximately 8.7 kDa. IP10 is secreted by several cell types such as monocytes endothelial cells and fibroblasts in response to interferon-gamma (IFN-γ). This protein is involved in immune responses and exhibits various roles especially in chemoattracting cells. Researchers often measure IP10 concentrations using ELISA kits such as the IP-10 ELISA to study its expression levels in different biological contexts.
- Biological function summary
IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.
- Pathways
The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.
- Associated diseases and disorders
IP10 is associated with conditions like multiple sclerosis and rheumatoid arthritis. Elevated IP10 levels often correlate with disease activity in these disorders making it a potential biomarker for disease progression. The protein interacts with other inflammatory mediators such as TNF-α in regulating immune activity within these disease contexts. IP10's involvement in recruiting immune cells contributes to the pathogenic inflammation observed in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Biotin Anti-IP10 antibody (ab271239)
Western blot analysis of varying levels of Recombinant rat IP10 protein using ab271239 at 0.2 μg/ml.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24All lanes: Western blot - Biotin Anti-IP10 antibody (ab271239)
Predicted band size: 10 kDa
Sandwich ELISA - Biotin Anti-IP10 antibody (ab271239)
Rat IP-10 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271239. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IP-10 protein.
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