Rabbit Monoclonal antibody - conjugated to Biotin. Suitable for IHC-P and reacts with samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Isotype Control [EPR25A] (AB320073), expandable thumbnail](https://content.abcam.com/products/images/biotin-isotype-control-epr25a-ab320073--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433888.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Isotype Control [EPR25A] (AB320073)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Version
Rabbit Monoclonal antibody - conjugated to Biotin. Suitable for IHC-P and reacts with samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- EPR25A
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Isotype Control [EPR25A] (ab320073), expandable thumbnail](https://content.abcam.com/products/images/biotin-isotype-control-epr25a-ab320073--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433888.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Isotype Control [EPR25A] (ab320073)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Isotype Control [EPR25A] (ab320073)
IHC image of formalin-fixed paraffin-embedded human breast cancer sections* tested on a Leica BONDTM.
Sections were pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using Avidin/Biotin Blocking Kit ab64212. The sections were then incubated with antibody (Biotin Anti-ISG15 antibody [EPR24482-49] ab317077, Rabbit monoclonal to ISG15 at 5ug/ml) or isotype control (ab320073, Rabbit IgG, at 5ug/ml) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX. The background control image is taken from an identical assay without primary antibody or isotype control.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com