AB271241

Biotin Anti-Klotho antibody

Name: Anti-Klotho Biotin antibody (ab271241) Rabbit polyclonal IgG | Abcam
Brand: Abcam Limited
SKU: AB271241

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(2 Publications)

Rabbit Polyclonal Klotho antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KL aa 1-550.

View Alternative Names

Klotho, KL

2 Images

Sandwich ELISA - Biotin Anti-Klotho antibody (AB271241)

sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-Klotho antibody (AB271241)

Human Klotho was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271241. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hKlotho protein.

Supplier Data

Western blot - Biotin Anti-Klotho antibody (AB271241)

Western blot analysis of varying levels of Recombinant human Klotho protein using ab271241 at 0.2 μg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant Klotho is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24

All lanes:

Western blot - Biotin Anti-Klotho antibody (ab271241)

Predicted band size: 116 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Applications

sELISA, WB

applications

Immunogen

Recombinant Fragment Protein within Human KL aa 1-550. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.

Purification technique

Affinity purification

Storage buffer

Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Klotho also known as alpha Klotho protein is a multifunctional protein prominently involved in regulating aging processes. It consists of approximately 130 kDa and is expressed mainly in the kidneys brain and parathyroid gland. This membrane-bound protein may also exist in a soluble form after cleavage by specific enzymes. Alpha Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) facilitating phosphate and vitamin D metabolism regulation.

Biological function summary

Alpha Klotho influences several cellular processes such as aging and mineral ion homeostasis. As a part of the FGF23-Klotho complex it enables the fibroblast growth factor to exert its biological effects. This protein modulates the effects of FGF23 on the renal excretion of phosphate and the synthesis of vitamin D. By interacting with FGF23 alpha Klotho supports maintaining electrolyte balance vital for proper physiological functioning.

Pathways

The FGF23-Klotho axis integrates into significant signaling pathways regulating mineral metabolism and aging. Klotho protein interactions with FGF23 contribute to controlling phosphate homeostasis by inhibiting renal phosphate reabsorption and suppressing vitamin D synthesis. It also engages with other proteins notably the fibroblast growth factors to regulate aging-related pathways ensuring cellular longevity and repair mechanisms are optimized.

Understanding Klotho's role helps in managing conditions like chronic kidney disease and some neurological disorders. Research indicates that reduced levels of alpha Klotho may correspond to kidney dysfunction due to its critical role in mineral metabolism. Furthermore alterations in Klotho expression connect with neurodegenerative diseases implicating its interaction with other proteins such as amyloid precursor protein and tau which are significant in disorders like Alzheimer's disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

See full target information KL

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell division 19:13 PubMed38632651

2024

Klotho-mediated activation of the anti-oxidant Nrf2/ARE signal pathway affects cell apoptosis, senescence and mobility in hypoxic human trophoblasts: involvement of Klotho in the pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Baomei Xu,Fang Cheng,Xiaolei Xue

Molecular and cellular biochemistry 476:1751-1763 PubMed33433832

2021

The circular RNA circSlc7a11 promotes bone cancer pain pathogenesis in rats by modulating LLC-WRC 256 cell proliferation and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Wen Chen,Xiao-Xia Zhang,Zhu-Ding Peng,Zu-Min Xing,Yi-Wen Zhang,Ya-Lan Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Klotho antibody

Sandwich ELISA - Biotin Anti-Klotho antibody (AB271241)

Western blot - Biotin Anti-Klotho antibody (AB271241)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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