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AB20766

Biotin Anti-Listeria monocytogenes antibody

4

(2 Reviews)

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(1 Publication)

Rabbit Polyclonal Listeria monocytogenes antibody - conjugated to Biotin. Suitable for ELISA, IHC-Fr and reacts with Listeria monocytogenes samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Listeria monocytogenes protein.

View Alternative Names

L. monocytogenes

1 Images
Immunohistochemistry (Frozen sections) - Biotin Anti-Listeria monocytogenes antibody (AB20766)
  • IHC-Fr

AbReview14348****

Immunohistochemistry (Frozen sections) - Biotin Anti-Listeria monocytogenes antibody (AB20766)

ab20766 staining Listeria monocytogenes in mouse spleen tissue section from a mice infected with 1x10exp6 L. monocytogenes for 24h by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 4% paraformaldehyde for 2 hours at RT and then it was incubated ON in 30% Sucrose in PBS at 4°C, froze away in OCT/Tissuetek. The tissue samples were blocked with 1% 1 : 20 Cd16/32 at 200C for 30 minutes. The sample was incubated with primary antibody (1/250) in PBS at 200C for 1 hour. For detection Streptavidin-Cy3 was used at dilution at 1 : 500 in PBS for 30 min at 20°C.

This image is a courtesy of Anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Listeria monocytogenes

Applications

IHC-Fr, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Listeria monocytogenes": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/1000", "ELISA-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/250", "IHCFr-species-notes": "<p></p>" } } }
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Product details

IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Listeria monocytogenes
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 5:e15567 PubMed21179567

2010

A fluorescence reporter model defines "Tip-DCs" as the cellular source of interferon β in murine listeriosis.

Applications

IHC-Fr

Species

Unspecified reactive species

Philipp Dresing,Stephanie Borkens,Magdalena Kocur,Sonja Kropp,Stefanie Scheu
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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