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AB271262

Biotin Anti-M-CSF/CSF1 antibody

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(1 Publication)

Goat Polyclonal M-CSF antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Csf1 aa 1-200.

View Alternative Names

Csfm, Csf1, Macrophage colony-stimulating factor 1, CSF-1, MCSF, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF

2 Images
Sandwich ELISA - Biotin Anti-M-CSF/CSF1 antibody (AB271262)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-M-CSF/CSF1 antibody (AB271262)

Mouse M-CSF was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271262. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mM-CSF protein.

Western blot - Biotin Anti-M-CSF/CSF1 antibody (AB271262)
  • WB

Supplier Data

Western blot - Biotin Anti-M-CSF/CSF1 antibody (AB271262)

Western blot analysis of varying levels of Recombinant murine M-CSF protein using ab271262 at 0.1 μg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant M-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24

All lanes:

Western blot - Biotin Anti-M-CSF/CSF1 antibody (ab271262)

Predicted band size: 60 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Mouse

Applications

sELISA, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Csf1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P07141

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Reactivity data

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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in PBS, 0.1% BSA
Purification technique
Affinity purification
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

M-CSF also called CSF1 or macrophage colony-stimulating factor is a cytokine with a molecular mass of approximately 40 kDa. It plays a significant role in the regulation of macrophage production differentiation and function. M-CSF is expressed in various tissues including the bone marrow spleen and liver. The expression of this cytokine is pivotal for the development and survival of mononuclear phagocytes and is critical in myeloid lineage regulation.
Biological function summary

This cytokine functions as a growth factor that influences the survival proliferation and differentiation of hematopoietic progenitor cells into mature macrophages. M-CSF forms a homodimeric complex that interacts with its receptor c-Fms which is a cell surface glycoprotein expressed on monocytes and macrophages. This interaction is essential for signal transduction that leads to the activation of pathways involved in cell growth and proliferation. Unlike some other growth factors M-CSF does not require the presence of a co-factor to exert its effects on target cells.

Pathways

M-CSF is involved in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are critical for the mediation of cell proliferation and survival. Within these pathways M-CSF connects with other proteins such as AKT1 and MAPK1 which further transduce signals that aid in cellular growth and immune response regulation. M-CSF also interacts through its receptor c-Fms which propagates downstream signaling ensuring the appropriate activation of macrophage-related pathways.

An imbalance or dysregulation of M-CSF activity has been linked to disorders such as osteoporosis and certain cancers. Osteoporosis involves excessive bone resorption due to increased osteoclast activity where M-CSF participates in osteoclast differentiation. In cancer particularly breast and prostate cancer M-CSF expression supports tumor-associated macrophages promoting tumor progression. Additionally c-Fms is involved in these disease processes creating a significant therapeutic target for modulating M-CSF activity to potentially alleviate symptoms or halt disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
See full target information Csf1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 12:851197 PubMed35651754

2022

E3 Ligase FBXW7 Facilitates Immune Evasion by Modulating TNF-α Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jingrui Song,Jin Chao,Xiaohong Hu,Xin Wen,Cairong Ding,Dan Li,Ding Zhang,Shanshan Han,Xiang Yu,Bo Yan,Zhu Jin,Yinhong Song,Jacqueline Gonzales,Laura E Via,Lu Zhang,Decheng Wang
View all publications
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Product promise

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