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AB17269

Biotin Anti-M13 antibody [E1]

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(1 Publication)

Mouse Monoclonal M13 antibody - conjugated to Biotin. Suitable for IHC, ELISA, Flow Cyt, WB and reacts with Enterobacteria phage M13 samples. Cited in 1 publication.

View Alternative Names

Bacteriophage M13, Coliphage M13, Enterobacteria phage M13, M13 virus, Phage M13

1 Images
ELISA - Biotin Anti-M13 antibody [E1] (AB17269)
  • ELISA

Supplier Data

ELISA - Biotin Anti-M13 antibody [E1] (AB17269)

ELISA absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of ab17269.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

E1

Isotype

IgG3

Light chain type

unknown

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Enterobacteria phage M13

Applications

Flow Cyt, ELISA, IHC, WB

applications

Specificity

M13 antibody [E1] (ab17269) is specific for gp3 protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Enterobacteria phage M13": { "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/mouse-igg2a-monoclonal-biotin-isotype-control-ab97679'>ab97679</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
M13 antibody [E1] (ab17269) is purified using 0.2µm sterile filter.
Storage buffer
Preservative: 0.08% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target 'M13' also known as M13 bacteriophage is a filamentous bacteriophage with a mass of approximately 2 MDa. This bacteriophage infects Escherichia coli bacteria and is characterized by a cylindrical shape with helical symmetry. The M13 phage expresses a protein coat made up of thousands of coat proteins including major coat protein g8p and minor proteins like g3p at its ends. These proteins interact with the bacterial surface for infection.
Biological function summary

M13 phage serves as a tool in phage display technology facilitating the identification of peptide-protein interactions. It does not actively participate in cellular processes but can be part of complex interactions in biotechnological applications. In contrast to lytic phages M13 allows for the continued survival of its host leading to its utilization in non-destructive recombinant DNA methodologies.

Pathways

M13 phage does not integrate into traditional metabolic or signaling pathways of the bacterial host. Instead it exploits the host's machinery for its replication and assembly. Not directly related to human biological pathways its use in phage display allows exploring interactions involving proteins such as antibodies or enzymes including the anti M13 HRP.

M13 phage does not directly relate to human diseases but it is essential in diagnostics and therapeutic antibody development. Researchers use phage display libraries to identify antibodies against targets involved in cancer and autoimmune diseases. The interaction between M13 and the Fc region of antibodies is exploited aiding in the precise selection of antibody fragments for therapeutic applications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information M13
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Methods in enzymology 70:266-74 PubMed7421592

1980

Use of the double-antibody method to separate antibody bound from free ligand in radioimmunoassay.

Applications

Unspecified application

Species

Unspecified reactive species

A R Midgley,M R Hepburn
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Product promise

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