Rabbit Polyclonal Macrophage Inflammatory Protein 1 gamma antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Mouse, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ccl9 aa 1 to C-terminus.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
Lyophilized
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Ccl9 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P51670
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Mouse | Expected | Tested |
| Recombinant full length protein - Mouse | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
Target data
Function
Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.
Alternative names
C-C motif chemokine 9, CCF18, Macrophage inflammatory protein 1-gamma, Macrophage inflammatory protein-related protein 2, Small-inducible cytokine A9, MIP-1-gamma, MRP-2, Mrp2, Scya10, Scya9, Ccl9
Recommended products
Rabbit Polyclonal Macrophage Inflammatory Protein 1 gamma antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Mouse, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ccl9 aa 1 to C-terminus.
Alternative names
C-C motif chemokine 9, CCF18, Macrophage inflammatory protein 1-gamma, Macrophage inflammatory protein-related protein 2, Small-inducible cytokine A9, MIP-1-gamma, MRP-2, Mrp2, Scya10, Scya9, Ccl9
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Ccl9 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P51670
- Purification technique
Affinity purification
- Reconstitution
Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
Macrophage Inflammatory Protein 1 gamma (MIP-1γ) also known as CCL9 functions as a chemokine involved in immune cell signaling. It weighs approximately 11 kDa. MIP-1γ attracts various immune cells to sites of inflammation within the body. The protein is expressed mainly in macrophages but you can also find it in other immune cells like dendritic cells. It plays a significant role in the body's response to inflammatory stimuli by mediating the recruitment of leukocytes.
- Biological function summary
MIP-1γ contributes to immune response modulation by acting as a signaling molecule. It helps coordinate the movement and activation of immune cells such as monocytes and lymphocytes thereby modulating inflammation. Unlike some other chemokines MIP-1γ does not usually form a complex with proteins. Its primary role involves direct interactions with specific receptors on target cells that initiate intracellular signaling pathways leading to cell migration and activation.
- Pathways
MIP-1γ engages in chemokine signaling and inflammatory response pathways. It intricately associates with the chemokine receptor CCR1 which is vital for mediating signal transduction processes essential for cell movement and immune surveillance. MIP-1γ also plays a role in the NF-kB signaling pathway important for transcription regulation and inflammatory response. This relationship highlights its involvement in orchestrating immune cell migration and the regulation of immune responses.
- Associated diseases and disorders
MIP-1γ connects to various conditions including chronic inflammatory diseases and certain cancers. For example it has a link to rheumatoid arthritis where it contributes to the persistence of inflammation by recruiting immune cells to affected joints. It is also associated with some types of leukemia where its expression levels can influence disease progression. Proteins like interleukin-1β interact with MIP-1γ in these conditions as they both participate in inflammatory responses that exacerbate disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Biotin Anti-Macrophage Inflammatory Protein 1 gamma antibody (ab271205)
To detect Murine MIP-1γ by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine MIP-1γ is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse Macrophage Inflammatory Protein 1 gamma, respectively.
Non-reducing conditions.
All lanes: Western blot - Biotin Anti-Macrophage Inflammatory Protein 1 gamma antibody (ab271205)
Sandwich ELISA - Biotin Anti-Macrophage Inflammatory Protein 1 gamma antibody (ab271205)
Mouse MIP-1γ was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271205.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mMIP-1γ protein.
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