Biotin Anti-Mast Cell Tryptase antibody [EPR9522]

2 product images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Mast Cell Tryptase antibody [EPR9522] (ab316948)

  IHC image of Mast Cell Tryptase staining in a section of formalin-fixed paraffin-embedded normal human colon*.

   Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using Avidin/Biotin Blocking Kit ab64212. The section was then incubated with ab316948 at 0.05 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Mast Cell Tryptase antibody [EPR9522] (ab316948)

  Negative staining of Mast Cell Tryptase in a section of formalin-fixed paraffin-embedded human normal skeletal muscle*.

  Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using Avidin/Biotin Blocking Kit ab64212. The section was then incubated with ab316948 at 0.05 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.