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AB316948

Biotin Anti-Mast Cell Tryptase antibody [EPR9522]

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Rabbit Recombinant Monoclonal TRYB1 antibody - conjugated to Biotin. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

TPS1, TPS2, TPSB1, TPSAB1, Tryptase alpha/beta-1, Tryptase-1, Tryptase I, Tryptase alpha-1, TPS2, TPSB2, Tryptase beta-2, Tryptase-2, Tryptase II

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Mast Cell Tryptase antibody [EPR9522] (AB316948)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Mast Cell Tryptase antibody [EPR9522] (AB316948)

Negative staining of Mast Cell Tryptase in a section of formalin-fixed paraffin-embedded human normal skeletal muscle*.

Performed on a Leica BONDTM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab316948 at 0.05 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Mast Cell Tryptase antibody [EPR9522] (AB316948)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Mast Cell Tryptase antibody [EPR9522] (AB316948)

IHC image of Mast Cell Tryptase staining in a section of formalin-fixed paraffin-embedded normal human colon*.

Performed on a Leica BONDTM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab316948 at 0.05 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Carrier free

    Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free

  • Carrier free

    Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free

  • Unconjugated

    Anti-Mast Cell Tryptase antibody [EPR9522]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Mast Cell Tryptase antibody [EPR9522]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Mast Cell Tryptase antibody [EPR9522]

  • HRP

    HRP Anti-Mast Cell Tryptase antibody [EPR9522]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9522

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB323931

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Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)

  • 0
  • 0
Primary Antibodies

AB280968

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  • 1
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Primary Antibodies

AB222113

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  • 0
  • 0
Primary Antibodies

AB306604

Alexa Fluor® 488 Anti-Periostin antibody [EPR20806]

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  • 0
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Proteins & Peptides

AB134529

Recombinant Human Mast Cell Tryptase protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human Mast Cell Tryptase protein (denatured) (His tag N-Terminus) (AB134529)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.05 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed : 18854315).
See full target information TPSAB1

Additional targets

TPSB2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com