Rabbit Polyclonal MCP1 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ccl2 aa 1 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
Lyophilized
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Ccl2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P10148
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Mouse | Predicted | Predicted |
| Recombinant fragment - Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed:29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed:29993042).
Alternative names
C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1, Mcp1, Je, Ccl2, Scya2
Recommended products
Rabbit Polyclonal MCP1 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ccl2 aa 1 to C-terminus.
Alternative names
C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1, Mcp1, Je, Ccl2, Scya2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Ccl2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P10148
- Purification technique
Affinity purification
- Reconstitution
Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
- Biological function summary
The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.
- Pathways
MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.
- Associated diseases and disorders
MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Biotin Anti-MCP1 antibody (ab271202)
To detect mouse MCP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant mouse MCP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse MCP1, respectively.
All lanes: Western blot - Biotin Anti-MCP1 antibody (ab271202)
Predicted band size: 11 kDa
Sandwich ELISA - Biotin Anti-MCP1 antibody (ab271202)
Mouse MCP1 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271202.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mouse MCP1 protein.
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