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AB271251

Biotin Anti-MCP2 antibody

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Rabbit Polyclonal MCP2 antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CCL8 aa 1 to C-terminus.

View Alternative Names

MCP2, SCYA10, SCYA8, CCL8, C-C motif chemokine 8, HC14, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, Small-inducible cytokine A8, MCP-2

2 Images
ELISA - Biotin Anti-MCP2 antibody (AB271251)
  • ELISA

Unknown

ELISA - Biotin Anti-MCP2 antibody (AB271251)

Human MCP2 was detected by sandwich ELISA (using 100 μl/well) using a concentration of 0.25-1.0 μg/ml of ab271251. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant MCP2 protein.

Western blot - Biotin Anti-MCP2 antibody (AB271251)
  • WB

Supplier Data

Western blot - Biotin Anti-MCP2 antibody (AB271251)

Western blot analysis of varying levels of Recombinant human MCP-2 protein using ab271251 at 0.1 μg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

All lanes:

Western blot - Biotin Anti-MCP2 antibody (ab271251)

Predicted band size: 11 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

ELISA, WB

applications

Immunogen

Recombinant Fragment Protein within Human CCL8 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P80075

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Primary Antibodies

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Proteins & Peptides

AB256074

Recombinant human MCP2 protein (Active)

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  • 0
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Primary Antibodies

AB18207

Anti-beta III Tubulin antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials|KO Validated

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.25-1 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Purification technique
Affinity purification
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP2 also known as CCL8 or monocyte chemoattractant protein-2 plays an important role in leukocyte trafficking. This chemokine has a molecular weight of approximately 8 kDa. CCL8 is expressed in various tissues including macrophages fibroblasts and endothelial cells and it is particularly high during inflammatory responses. The MCP2 protein attracts immune cells such as monocytes and T cells to sites of infection or injury.
Biological function summary

MCP2 influences the migration and activation of leukocytes enhancing the body's immune response. MCP2 interacts with chemokine receptors like CCR2 and CCR5 facilitating the movement of immune cells through tissues. This protein is not usually part of larger complexes but works closely with other chemokines to coordinate cellular responses during inflammation. MCP2 activity is important in processes that require rapid cell recruitment and immune surveillance.

Pathways

MCP2 functions within the chemokine signaling pathway and plays a significant part in the inflammatory response pathway. MCP2 closely interacts with chemokine receptors such as CCR2 which is also engaged by related chemokines like MCP1 (CCL2). MCP2 and MCP1 share functional similarities in modulating the immune response though they target different receptor profiles that expand their influence across numerous immune processes.

MCP2 is associated with inflammatory diseases and certain types of cancer. Rheumatoid arthritis shows heightened levels of MCP2 contributing to the persistent inflammation characteristic of the disorder. Elevated MCP2 levels have also been noticed in tumor microenvironments suggesting a role in cancer metastasis where it might interact with proteins such as IL-6 to promote the survival and migration of tumor cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
See full target information CCL8
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Product promise

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For full details, please see our Terms & Conditions

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