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Goat Polyclonal MCP4 antibody - conjugated to Biotin. Suitable for WB and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CCL13 aa 1 to C-terminus.

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Images

Western blot - Biotin Anti-MCP4 antibody (AB271198), expandable thumbnail

Key facts

Isotype

IgG

Host species

Goat

Conjugation

Biotin

Storage buffer

Constituents: PBS, 0.1% BSA

Form

Lyophilized

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human CCL13 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99616

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Predicted
Recombinant full length protein - Human
Tested

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Human

Dilution info

-

Notes

-

Target data

Function

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

Alternative names

Recommended products

Goat Polyclonal MCP4 antibody - conjugated to Biotin. Suitable for WB and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CCL13 aa 1 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Conjugation

Biotin

Form

Lyophilized

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human CCL13 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99616
Purification technique

Affinity purification

Reconstitution

Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MCP4 also known as CCL13 is a chemokine with a molecular mass of approximately 11 kDa. It plays a critical role in attracting immune cells to sites of inflammation. MCP4 achieves this through its interaction with G protein-coupled receptors particularly CCR2 and CCR3. The protein is primarily expressed in tissues involved in immune responses including lymph nodes spleen and bone marrow. It exhibits high expression levels at sites of allergic inflammation suggesting a specific role in these processes.

Biological function summary

MCP4 functions by mediating the recruitment of monocytes eosinophils and T lymphocytes. It is not part of a larger protein complex but rather operates as part of the chemokine signaling family. MCP4 is particularly active in coordinating responses to allergens and pathogens therefore highlighting its importance in the body's defense mechanisms. As a member of the C-C motif chemokine family it shares structural characteristics with other chemokines like CCL11 which participate in similar immune cell trafficking functions.

Pathways

MCP4 is deeply involved in inflammatory and immune pathways specifically the chemokine signaling pathway and the cytokine-cytokine receptor interaction pathway. MCP4 guides immune cells allowing them to respond effectively to cytokine signals ensuring their migration to necessary sites. Through the chemokine signaling pathway it collaborates with related chemokines like CCL5 to enhance immune responses. Its interaction with these pathways highlights its role in both routine immunity and inflammatory responses positioning it as an important player in maintaining immune homeostasis.

Associated diseases and disorders

MCP4 correlates with conditions such as asthma and allergic rhinitis. Both of these conditions involve chronic inflammation where MCP4's role becomes more pronounced. In asthma the protein contributes to the recruitment of eosinophils and other immune cells that drive the inflammatory processes. MCP4 interactions with proteins like CCR3 are significant in exacerbating symptoms of allergic disorders making it an important target for therapeutic research aimed at modulating inflammatory responses in these diseases.

Product promise

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1 product image

  • Western blot - Biotin Anti-MCP4 antibody (ab271198), expandable thumbnail

    Western blot - Biotin Anti-MCP4 antibody (ab271198)

    To detect Human MCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml.

    When used in conjunction with compatible development reagents the detection limit for Recombinant Human MCP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

    Lane 1: Marker

    Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human MCP4, respectively.

    Non-reducing conditions.

    All lanes: Western blot - Biotin Anti-MCP4 antibody (ab271198)

    Predicted band size: 11 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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