JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB93528

Biotin Anti-MHC class I antibody [AF6-88.5.5.3]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal HA1W antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing H2-K1 protein.

View Alternative Names

H2-K

1 Images
Flow Cytometry - Biotin Anti-MHC class I antibody [AF6-88.5.5.3] (AB93528)
  • Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-MHC class I antibody [AF6-88.5.5.3] (AB93528)

Staining of C57Bl/6 splenocytes with 0.06µg of biotin Mouse IgG2a, K Isotype control (left) or 0.06 µg of ab93528(right), followed by Streptavidin-PE.
Total viable cells were used for analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AF6-88.5.5.3

Isotype

IgG2a

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing H2-K1 protein. The exact immunogen used to generate this antibody is proprietary information.

P03991

Specificity

Reacts with the H-2Kb MHC class I alloantigen. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.06 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/mouse-igg2a-monoclonal-biotin-isotype-control-ab97679'>ab97679</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MHC class I also known as MHC-1 or simply MHC I is a molecule playing an important role in the immune system. The molecule is a heterodimer composed of a heavy chain weighing around 45 kDa and a light chain. MHC class I molecules are expressed on nearly all nucleated cells in the body. They present peptide fragments from intracellular proteins to CD8+ T cells helping the immune system recognize and destroy infected or cancerous cells. Alternate names like H-2Kd/H-2Dd in mice highlight its role in antigen presentation across different species.
Biological function summary

The molecules of MHC class I form part of the adaptive immune system. They act as a complex with peptides which are derived from proteins degraded by the proteasome. The peptide-MHC I complex travels to the cell surface where it engages with the T cell receptor (TCR) on cytotoxic T cells. This interaction is essential for immune surveillance ensuring the body identifies cells that might compromise health such as those infected by viruses or transformed malignantly.

Pathways

The function of MHC class I integrates into the antigen processing and presentation pathways. It is pivotal in the proteasome-dependent pathway for processing endogenous antigens. Proteins like TAP (transporter associated with antigen processing) assist in transporting peptides into the endoplasmic reticulum for loading onto MHC class I. This pathway is central to cellular immunity and intersects with various immune response cascades.

MHC class I has associations with autoimmune disorders and certain viral infections. Aberrant expression or mutations can lead to diseases like Ankylosing Spondylitis or impact responses to chronic viral infections such as HIV. The involvement of MHC class I in presenting viral peptides often links it to immune evasion mechanisms utilizable by viruses. Hence this protein works closely with immune system proteins like cytokines influencing disease outcomes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the presentation of foreign antigens to the immune system.
See full target information H2-K1

Additional targets

H2-K1,,H2-K1,H2-K1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced therapeutics 3: PubMed34141865

2020

An Antigen-Delivery Protein Nanoparticle Combined with Anti-PD-1 Checkpoint Inhibitor Has Curative Efficacy in an Aggressive Melanoma Model.

Applications

Unspecified application

Species

Unspecified reactive species

Medea Neek,Jo Anne Tucker,Nina Butkovich,Edward L Nelson,Szu-Wen Wang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB95572

FITC Anti-MHC class I antibody [34-1-2S]

Flow Cytometry - FITC Anti-MHC class I antibody [34-1-2S] (AB95572)

  • 0
  • 0
Primary Antibodies

AB171283

Anti-MHC class I antibody [28-14-8] - Low endotoxin, Azide free

  • 0
  • 0
Primary Antibodies

AB95571

PE Anti-MHC class I antibody [34-1-2S]

Flow Cytometry - PE Anti-MHC class I antibody [34-1-2S] (AB95571)

  • 0
  • 0
Primary Antibodies

AB244556

Anti-MHC class I antibody [OX18] - BSA and Azide free

Recombinant

Immunohistochemistry (Frozen sections) - Anti-MHC class I antibody [OX18] - BSA and Azide free (AB244556)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB95572

FITC Anti-MHC class I antibody [34-1-2S]

Flow Cytometry - FITC Anti-MHC class I antibody [34-1-2S] (AB95572)

  • 0
  • 0
Primary Antibodies

AB52922

Anti-HLA A antibody [EP1395Y]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-HLA A antibody [EP1395Y] (AB52922)

  • 4
  • 15
Primary Antibodies

AB267388

Anti-Thrombospondin 1 antibody [EPR22927-54]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22927-54] (AB267388)

  • 2
  • 1
Primary Antibodies

AB305103

Anti-TREM2 antibody [EPR26210-1]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR26210-1] (AB305103)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com