AB222764

Biotin Anti-Mouse IgG2a antibody [RM219]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal GCAA antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Mouse Ighg.

View Alternative Names

Immunoglobulin heavy chain gamma polypeptide, Ighg

3 Images

ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (AB222764)

ELISA

Supplier Data

ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (AB222764)

ELISA of mouse immunoglobulins shows ab222764 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab222764 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

Supplier Data

Western blot - Biotin Anti-Mouse IgG2a antibody [RM219] (AB222764)

This antibody reacts to non-reduced IgG2a ~150 kDa.

All lanes:

Western blot - Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764) at 0.5 µg/mL

Lane 1:

Non-reduced mouse IgG2a

Lane 2:

Reduced mouse IgG2a

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ELISA

Supplier Data

ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (AB222764)

A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of ab222764 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM219

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Mouse

Applications

ELISA, WB

applications

Immunogen

Full Length Protein corresponding to Mouse Ighg. The exact immunogen used to generate this antibody is proprietary information.

P01863

Specificity

ab222764 reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.005-0.2 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab222764 was purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mouse IgG2a also known as immunoglobulin G subclass 2a is an antibody isotype found in mice. It plays an essential role in the immune response by recognizing and binding antigens. IgG2a has a molecular mass of approximately 150 kDa. It is predominantly expressed in plasma cells and can be found in the serum. This antibody class is frequently used in immunological research to study immune system function in mouse models.

Biological function summary

IgG2a performs an important role in mediating antibody-dependent cellular cytotoxicity (ADCC) and complement activation. It is part of the immunoglobulin complex and is important for targeting viral and bacterial pathogens. Mouse IgG2a antibodies engage Fc receptors on effector cells such as natural killer cells and macrophages initiating immune response and promoting the clearance of pathogens.

Pathways

This antibody isotype actively participates in humoral immunity pathways. The Fc gamma receptor pathway is particularly important as it facilitates effector functions like phagocytosis and ADCC. IgG2a shares pathways with related proteins including other immunoglobulin subclasses like IgG1 influencing these immune mechanisms in different but complementary ways.

Mouse IgG2a is relevant in autoimmune disease models and infectious disease research. Its role is significant in conditions like lupus erythematosus and rheumatoid arthritis where pathogenic antibody production occurs. IgG2a along with other immunoglobulin subclasses interacts with proteins like cytokines which influence disease progression and immune regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7694 PubMed39227587

2024

Phosphorylation activates master growth regulator DELLA by promoting histone H2A binding at chromatin in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Huang,Rodolfo Zentella,Jeongmoo Park,Larry Reser,Dina L Bai,Mark M Ross,Jeffrey Shabanowitz,Donald F Hunt,Tai-Ping Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Mouse IgG2a antibody [RM219]

ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (AB222764)

Western blot - Biotin Anti-Mouse IgG2a antibody [RM219] (AB222764)

ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (AB222764)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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