AB197139

Biotin Anti-Myc tag antibody [9E10] - C-terminal

Name: Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139) | Abcam
Brand: Abcam Limited
SKU: AB197139

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(2 Publications)

Mouse Monoclonal C-Myc/MYC antibody - conjugated to Biotin. C-terminal. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Tag samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MYC aa 400 to C-terminus.

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

1 Images

Flow Cytometry (Intracellular) - Biotin Anti-Myc tag antibody [9E10] - C-terminal (AB197139)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Biotin Anti-Myc tag antibody [9E10] - C-terminal (AB197139)

Flow cytometry intracellular staining pattern of LST-1-c-Myc transfected HEK-293 cells stained using ab197139 (concentration in sample 5 µg/ml, Streptavidin PE).

617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Myc tag antibody [9E10]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Myc tag antibody [9E10]
660 APC

APC Anti-Myc tag antibody [9E10]
HRP

HRP Anti-Myc tag antibody [9E10]
578 PE

PE Anti-Myc tag antibody [9E10]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Myc tag antibody [9E10]
Unconjugated

Anti-Myc tag antibody [9E10]
657 SureLight® APC

SureLight® APC Anti-Myc tag antibody [9E10]
576 DyLight® 550

DyLight® 550 Anti-Myc tag antibody [9E10]
673 DyLight® 650

DyLight® 650 Anti-Myc tag antibody [9E10]
518 DyLight® 488

DyLight® 488 Anti-Myc tag antibody [9E10]
519 FITC

FITC Anti-Myc tag antibody [9E10]
Biotin

Biotin Anti-Myc tag antibody [9E10]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9E10

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission

Carrier free

Applications

Flow Cyt, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human MYC aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P01106

Specificity

This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/biotin-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18434'>ab18434</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1-5 µg/mL", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/biotin-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18434'>ab18434</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Storage buffer

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).

See full target information MYC

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:10964 PubMed38744871

2024

Stem cells derived exosomes as biological nano carriers for VCR sulfate for treating breast cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed H Farouk,Ahmed Aref,Belal A Fathy,Ahmed N Abdallah

Science advances 8:eabo7792 PubMed36054348

2022

Genome-wide in vivo screen of circulating tumor cells identifies as a regulator of metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Xia,Yuan Ma,Kangfu Chen,Bill Duong,Sharif Ahmed,Randy Atwal,David Philpott,Troy Ketela,Jennifer Pantea,Sichun Lin,Stephane Angers,Shana O Kelley

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Myc tag antibody [9E10] - C-terminal

Flow Cytometry (Intracellular) - Biotin Anti-Myc tag antibody [9E10] - C-terminal (AB197139)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise