Mouse Monoclonal C-Myc/MYC antibody - conjugated to Biotin. C-terminal. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Tag samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MYC aa 400 to C-terminus.
Images
![Flow Cytometry (Intracellular) - Biotin Anti-Myc tag antibody [9E10] - C-terminal (AB197139), expandable thumbnail](https://content.abcam.com/products/images/biotin-myc-tag-antibody-9e10-c-terminal-ab197139--flow-cytometry-intracellular-img439020.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Biotin Anti-Myc tag antibody [9E10] - C-terminal (AB197139)")
Publications
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- Science advances 8:eabo77922022Genome-wide in vivo screen of circulating tumor cells identifies as a regulator of metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Xia,Yuan Ma,Kangfu Chen,Bill Duong,Sharif Ahmed,Randy Atwal,David Philpott,Troy Ketela,Jennifer Pantea,Sichun Lin,Stephane Angers,Shana O KelleyPubMed 36054348
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Biotin
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human MYC aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P01106
Reactivity data
Select an application| Flow Cyt | Flow Cyt (Intra) | |
|---|---|---|
| Tag | Expected | Tested |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1.00000-5.00000 µg/mL | Notes ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1.00000-5.00000 µg/mL | Notes ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
14 products for Alternative Version
Target data
Function
Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
Alternative names
BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39
Recommended products
Mouse Monoclonal C-Myc/MYC antibody - conjugated to Biotin. C-terminal. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Tag samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MYC aa 400 to C-terminus.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Biotin
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human MYC aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P01106
- Clone number
- 9E10
- Purification technique
- Size-exclusion chromatography
- Specificity
This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc.
- Concentration
- Purification notes
Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Flow Cytometry (Intracellular) - Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139), expandable thumbnail](https://content.abcam.com/products/images/biotin-myc-tag-antibody-9e10-c-terminal-ab197139--flow-cytometry-intracellular-img439020.jpg "Flow Cytometry (Intracellular) - Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)")
Flow Cytometry (Intracellular) - Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)
Flow cytometry intracellular staining pattern of LST-1-c-Myc transfected HEK-293 cells stained using ab197139 (concentration in sample 5 µg/ml, Streptavidin PE).
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