AB34773

Biotin Anti-Myc tag antibody

Name: Anti-Myc tag Biotin antibody (ab34773) Rabbit polyclonal IgG | Abcam
Brand: Abcam Limited
SKU: AB34773
Rating: 5 (3 reviews)

(3 Reviews)

(2 Publications)

Rabbit Polyclonal Myc tag antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Tag samples. Cited in 2 publications.

View Alternative Names

Myc Epitope Tag, c-myc tag

1 Images

AbReview13751****

Western blot - Biotin Anti-Myc tag antibody (AB34773)

Mouse NIH 3T3 cells over-expressing pGEN IRES pyk2 Myc-tag. ab34773 was used at 1/2500 dilution. Please see accompanying abreview for additional information.

All lanes:

Western blot - Biotin Anti-Myc tag antibody (ab34773)

Predicted band size: 49 kDa

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This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Applications

ELISA, WB

applications

Specificity

This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxytermini of targeted proteins in transfected or transformed cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/100000 - 1/500000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Myc tag

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:1673 PubMed30975984

2019

Microtubule end conversion mediated by motors and diffusing proteins with no intrinsic microtubule end-binding activity.

Applications

Unspecified application

Species

Unspecified reactive species

Manas Chakraborty,Ekaterina V Tarasovetc,Anatoly V Zaytsev,Maxim Godzi,Ana C Figueiredo,Fazly I Ataullakhanov,Ekaterina L Grishchuk

Molecular and cellular biology 33:4017-29 PubMed23938296

2013

Pdx-1 activates islet α- and β-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2.

Applications

Unspecified application

Species

Unspecified reactive species

Heather L Hayes,Larry G Moss,Jonathan C Schisler,Jonathan M Haldeman,Zhushan Zhang,Paul B Rosenberg,Christopher B Newgard,Hans E Hohmeier

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Myc tag antibody

Western blot - Biotin Anti-Myc tag antibody (AB34773)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

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