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AB212021

Biotin Anti-MyD88 antibody

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(1 Publication)

Goat Polyclonal MYD88 antibody - conjugated to Biotin. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MYD88 aa 250 to C-terminus.

View Alternative Names

Myeloid differentiation primary response protein MyD88, MYD88

1 Images
Western blot - Biotin Anti-MyD88 antibody (AB212021)
  • WB

Supplier Data

Western blot - Biotin Anti-MyD88 antibody (AB212021)

Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

All lanes:

Western blot - Biotin Anti-MyD88 antibody (ab212021) at 0.2 µg/mL

All lanes:

Human thymus lysate (in RIPA buffer) at 35 µg

Predicted band size: 33 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MYD88 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99836

Specificity

ab212021 is expected to recognize reported isoforms 1, 2 and 3 (NP_001166038.1; NP_002459.2; NP_001166039.1).

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Alternative Products

Primary Antibodies

AB218705

HRP Anti-MyD88 antibody [EPR590(N)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-MyD88 antibody [EPR590(N)] (AB218705)

  • 0
  • 0
Primary Antibodies

AB133739

Anti-MyD88 antibody [EPR590(N)]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [EPR590(N)] (AB133739)

  • 5
  • 1
Primary Antibodies

AB219413

Anti-MyD88 antibody [EPR21824]

RabMAb|Recombinant|KO Validated

Western blot - Anti-MyD88 antibody [EPR21824] (AB219413)

  • 0
  • 0
Primary Antibodies

AB199247

Anti-MyD88 antibody [EPR590(N)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [EPR590(N)] - BSA and Azide free (AB199247)

  • 0
  • 0
Primary Antibodies

AB236766

Anti-MyD88 antibody [EPR21824] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-MyD88 antibody [EPR21824] - BSA and Azide free (AB236766)

  • 0
  • 0

Complementary Products

Assay Kits

AB171341

Human MyD88 ELISA Kit

Sandwich ELISA - Human MyD88 ELISA Kit (AB171341)

  • 0
  • 0
Proteins & Peptides

AB114217

Recombinant Human MyD88 protein

SDS-PAGE - Recombinant Human MyD88 protein (AB114217)

  • 0
  • 0
Assay Kits

AB171341

Human MyD88 ELISA Kit

Sandwich ELISA - Human MyD88 ELISA Kit (AB171341)

  • 0
  • 0
Primary Antibodies

AB22048

Anti-TLR4 antibody [76B357.1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR4 antibody [76B357.1] (AB22048)

  • 4
  • 29
Primary Antibodies

AB168382

Anti-TLR5 antibody [EPR10373]

RabMAb|Recombinant

Western blot - Anti-TLR5 antibody [EPR10373] (AB168382)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab212021 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MyD88 standing for myeloid differentiation primary response 88 is a cytoplasmic adaptor protein with a molecular weight of approximately 33 kDa. This protein is expressed widely in immune cells including monocytes macrophages and dendritic cells. MyD88 serves a major role in the signaling pathways for the innate immune system. It acts as a linker transmitting signals from toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) to downstream signaling molecules ultimately activating transcription factors.
Biological function summary

MyD88 plays a significant role in mediating immune responses by forming part of a complex that includes IRAK kinases and TRAF6. When TLRs or IL-1Rs activate MyD88 this adaptor protein recruits IRAK4 which then phosphorylates IRAK1 or IRAK2. This cascade promotes the activation of NF-κB and MAPK pathways leading to the production of inflammatory cytokines. The MyD88-dependent pathway is integral to innate immunity influencing how the body responds to pathogen infection and inflammation.

Pathways

MyD88 integrates into both the TLR signaling and IL-1R signaling pathways. Key related proteins in these pathways include interleukin-1 receptor-associated kinase (IRAK) and tumor necrosis factor receptor-associated factor 6 (TRAF6). MyD88 initiates the recruitment and activation of IRAK1 and IRAK4 following receptor engagement leading to subsequent activation of downstream signals. As part of these pathways MyD88 mediates cellular responses important for immune system signaling and inflammatory response regulation.

MyD88 involvement is notable in the context of oncological and autoimmune diseases. Mutations or dysregulation of MyD88 are implicated in conditions like lymphoma and rheumatoid arthritis. In lymphoma the mutation usually results in constant activation of NF-κB leading to unchecked cell growth. As for rheumatoid arthritis an overactive immune response due to MyD88 can cause additional inflammation affecting joint tissues. In these conditions MyD88 interaction with IRAK4 is significant given their mutual role in immune and inflammatory pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed : 15361868, PubMed : 18292575, PubMed : 33718825, PubMed : 37971847). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 15361868, PubMed : 19506249, PubMed : 24316379, PubMed : 40638072). Increases IL-8 transcription (PubMed : 9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation (PubMed : 33718825). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).
See full target information MYD88
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Virology journal 21:253 PubMed39385182

2024

Upregulation of keratin 15 is required for varicella-zoster virus replication in keratinocytes and is attenuated in the live attenuated vOka vaccine strain.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Tommasi,Ohad Yogev,Michael B Yee,Andriani Drousioti,Meleri Jones,Alice Ring,Manuraj Singh,Inga Dry,Oscar Atkins,Aishath S Naeem,Nisha Kriplani,Arne N Akbar,Jürgen G Haas,Edel A O'Toole,Paul R Kinchington,Judith Breuer
View all publications
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Product promise

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