AB93524

Biotin Anti-N Cadherin antibody [8C11]

Name: Anti-N Cadherin Biotin antibody [8C11] (ab93524) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB93524

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(3 Publications)

Mouse Monoclonal N Cadherin antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Fusion protein corresponding to aa 50-600 of CDH2, fused to CDH2.

View Alternative Names

CD325, CDHN, NCAD, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin

1 Images

Flow Cytometry - Biotin Anti-N Cadherin antibody [8C11] (AB93524)

Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-N Cadherin antibody [8C11] (AB93524)

Flow Cytometry staining of Hela cell line with Biotin Mouse IgG1, K Isotype Control (blue histogram) or ab93524 (purple histogram) followed by Streptavidin PE. Total viable cells were used for analysis.

Unconjugated

Anti-N Cadherin antibody [8C11]
Carrier free

Anti-N Cadherin antibody [8C11] - BSA and Azide free
578 PE

PE Anti-N Cadherin antibody [8C11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8C11

Isotype

IgG1

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Fusion protein corresponding to aa 50-600 of CDH2, fused to CDH2. The exact immunogen used to generate this antibody is proprietary information.

P19022

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.25 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/biotin-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18434'>ab18434</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone : upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

See full target information CDH2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Small (Weinheim an der Bergstrasse, Germany) 16:e1905614 PubMed32141228

2020

Tracking Drug-Induced Epithelial-Mesenchymal Transition in Breast Cancer by a Microfluidic Surface-Enhanced Raman Spectroscopy Immunoassay.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Jing Wang,Karthik Balaji Shanmugasundaram,Belinda Yeo,Andreas Möller,Alain Wuethrich,Lynlee L Lin,Matt Trau

The Journal of biological chemistry 278:17269-76 PubMed12604612

2003

N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.

Applications

Species

Unspecified reactive species

James K Wahl,Young J Kim,Janet M Cullen,Keith R Johnson,Margaret J Wheelock

Journal of cell science 114:1567-77 PubMed11282032

2001

N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation.

Applications

Flow Cyt

Species

Unspecified reactive species

S Puch,S Armeanu,C Kibler,K R Johnson,C A Müller,M J Wheelock,G Klein

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Biotin Anti-N Cadherin antibody [8C11]

Flow Cytometry - Biotin Anti-N Cadherin antibody [8C11] (AB93524)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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