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AB240487

Biotin Anti-Neurogranin antibody

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(1 Publication)

Goat Polyclonal Neurogranin antibody - conjugated to Biotin. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NRGN aa 1 to C-terminus.

View Alternative Names

Neurogranin, Ng, RC3, NRGN

1 Images
Western blot - Biotin Anti-Neurogranin antibody (AB240487)
  • WB

Supplier Data

Western blot - Biotin Anti-Neurogranin antibody (AB240487)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Biotin Anti-Neurogranin antibody (ab240487) at 0.1 µg/mL

All lanes:

Human brain (Frontal cortex) lysate in RIPA buffer at 35 µg

Predicted band size: 8 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human NRGN aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92686

Specificity

Reported variants represent identical protein: NP_006167.1, NP_001119653.1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Goat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurogranin also known as NRGN is a small neuronal protein with an approximate molecular mass of 7.6 kDa. It is mainly expressed in the brain predominantly within the dendrites of neurons. Neurogranin binds to calmodulin in the presence of calcium playing an important role in synaptic plasticity. This protein acts as a kinase substrate influencing signal transduction pathways tied to learning and memory. Due to its specific expression pattern neurogranin serves as a significant molecular marker for studying the nervous system.
Biological function summary

Neurogranin functions as a modulator in cerebellar signaling and synaptic transmission. It influences calcium-calmodulin signaling facilitating the activation of proteins important in the central nervous system. Neurogranin does not form part of a multi-protein complex but interacts closely with calmodulin to regulate downstream signal cascades. These interactions emphasize its fundamental role in modulating processes that influence cognitive functions like learning and memory.

Pathways

Neurogranin significantly impacts the calcium signaling pathway and contributes to the regulation of long-term potentiation. It closely associates with proteins such as Protein Kinase C (PKC) and calmodulin-dependent protein kinases which are instrumental in these pathways. By modulating these essential signaling routes neurogranin affects intracellular signals that are vital for synaptic plasticity and enhanced neural communication.

Neurogranin has a noted involvement in conditions like Alzheimer's disease and cognitive impairment. Changes in neurogranin expression levels are linked to the progression of Alzheimer's where reduced levels may contribute to the disruption of synaptic plasticity. Interactions with amyloid-beta peptides and other proteins such as tau highlight its potential as a biomarker for neurodegenerative diseases. These connections underline neurogranin's significant role in understanding cognitive-related disorders and guiding therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium (By similarity).
See full target information NRGN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 15:415 PubMed39533309

2024

Lactylation of Hdac1 regulated by Ldh prevents the pluripotent-to-2C state conversion.

Applications

Unspecified application

Species

Unspecified reactive species

Qiman Dong,Xiaoqiong Yang,Lingling Wang,Qingye Zhang,Nannan Zhao,Shanshan Nai,Xiaoling Du,Lingyi Chen
View all publications
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