Rabbit Polyclonal Neurotrophin 3 antibody - conjugated to Biotin. Suitable for ELISA and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NTF3 aa 100 to C-terminus.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
Lyophilized
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NTF3 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20783
Reactivity data
Select an application| ELISA | |
|---|---|
| Recombinant fragment - Human | Tested |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 0.25000-1.00000 µg/mL | Notes Human NT-3was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271274. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hNT-3 protein. |
Target data
Function
Seems to promote the survival of visceral and proprioceptive sensory neurons.
Alternative names
Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, Neurotrophic factor, NGF-2, NTF3
Recommended products
Rabbit Polyclonal Neurotrophin 3 antibody - conjugated to Biotin. Suitable for ELISA and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NTF3 aa 100 to C-terminus.
Alternative names
Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, Neurotrophic factor, NGF-2, NTF3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NTF3 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20783
- Purification technique
Affinity purification
- Reconstitution
Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
Neurotrophin 3 also known as NT-3 or neurotrophin-3 is a protein with a molecular mass around 28 kDa. It belongs to the neurotrophin family and acts mechanically by binding to specific tyrosine kinase receptors especially TrkC on target cells. This binding initiates intracellular signaling cascades essential for neuronal development and survival. NT-3 is expressed in the central and peripheral nervous systems and is involved in various cellular processes. Expression levels can vary depending upon developmental stages and types of nervous tissue.
- Biological function summary
NT-3 has a role in the survival and differentiation of neurons especially sensory ganglia neurons. It supports the development and maintenance of the nervous system by influencing neuron growth and plasticity. While not typically forming a large protein complex NT-3 interacts with its receptor TrkC to transmit critical signals internally. Such interactions can modulate synaptic responses and connectivity ultimately influencing learning and memory formation. NT-3 also interacts with p75NTR another receptor though this interaction is less specific compared to its action on TrkC.
- Pathways
NT-3 participates in the MAPK/ERK signaling and PI3K/AKT pathways. These pathways are fundamental in regulating cell differentiation growth and survival. They also mediate a variety of cellular responses to external stimuli. NT-3's activation of the TrkC receptor triggers these pathways affecting other proteins such as BDNF (brain-derived neurotrophic factor) and NGF (nerve growth factor) which are part of the neurotrophin family. The intricate web of interactions highlights NT-3's significance in pathway modulation involved in neurodevelopment and plasticity.
- Associated diseases and disorders
NT-3 has implications in neurodegenerative diseases like Parkinson’s disease and Huntington's disease. The protein’s role in supporting neuronal survival points to its potential impact when its signaling is disrupted or deficient. NT-3 exhibits connections with proteins such as TrkC and BDNF which are also related to these neurodegenerative disorders. Dysfunctional NT-3-TrkC signaling could contribute to disease progression by impairing neuronal survival or recovery. Understanding NT-3's role in these contexts can offer insights into therapeutic approaches for such conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
ELISA - Biotin Anti-Neurotrophin 3 antibody (ab271274)
Human Neurotrophin 3 was detected by sandwich ELISA (using 100 μl/well) using a concentration of 0.25-1.0 μg/ml of ab271274. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Neurotrophin 3 protein.
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