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Rabbit Polyclonal Neurotrophin 3 antibody - conjugated to Biotin. Suitable for ELISA and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NTF3 aa 100 to C-terminus.

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Images

ELISA - Biotin Anti-Neurotrophin 3 antibody (AB271274), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Biotin

Storage buffer

Constituents: PBS, 0.1% BSA

Form

Lyophilized

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human NTF3 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20783

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISA
Recombinant fragment - Human
Tested

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

0.25000-1.00000 µg/mL

Notes

Human NT-3was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271274. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hNT-3 protein.

Target data

Function

Seems to promote the survival of visceral and proprioceptive sensory neurons.

Alternative names

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Rabbit Polyclonal Neurotrophin 3 antibody - conjugated to Biotin. Suitable for ELISA and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NTF3 aa 100 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Conjugation

Biotin

Form

Lyophilized

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human NTF3 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20783
Purification technique

Affinity purification

Reconstitution

Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Activity summary

Neurotrophin 3 also known as NT-3 or neurotrophin-3 is a protein with a molecular mass around 28 kDa. It belongs to the neurotrophin family and acts mechanically by binding to specific tyrosine kinase receptors especially TrkC on target cells. This binding initiates intracellular signaling cascades essential for neuronal development and survival. NT-3 is expressed in the central and peripheral nervous systems and is involved in various cellular processes. Expression levels can vary depending upon developmental stages and types of nervous tissue.

Biological function summary

NT-3 has a role in the survival and differentiation of neurons especially sensory ganglia neurons. It supports the development and maintenance of the nervous system by influencing neuron growth and plasticity. While not typically forming a large protein complex NT-3 interacts with its receptor TrkC to transmit critical signals internally. Such interactions can modulate synaptic responses and connectivity ultimately influencing learning and memory formation. NT-3 also interacts with p75NTR another receptor though this interaction is less specific compared to its action on TrkC.

Pathways

NT-3 participates in the MAPK/ERK signaling and PI3K/AKT pathways. These pathways are fundamental in regulating cell differentiation growth and survival. They also mediate a variety of cellular responses to external stimuli. NT-3's activation of the TrkC receptor triggers these pathways affecting other proteins such as BDNF (brain-derived neurotrophic factor) and NGF (nerve growth factor) which are part of the neurotrophin family. The intricate web of interactions highlights NT-3's significance in pathway modulation involved in neurodevelopment and plasticity.

Associated diseases and disorders

NT-3 has implications in neurodegenerative diseases like Parkinson’s disease and Huntington's disease. The protein’s role in supporting neuronal survival points to its potential impact when its signaling is disrupted or deficient. NT-3 exhibits connections with proteins such as TrkC and BDNF which are also related to these neurodegenerative disorders. Dysfunctional NT-3-TrkC signaling could contribute to disease progression by impairing neuronal survival or recovery. Understanding NT-3's role in these contexts can offer insights into therapeutic approaches for such conditions.

Product promise

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1 product image

  • ELISA - Biotin Anti-Neurotrophin 3 antibody (ab271274), expandable thumbnail

    ELISA - Biotin Anti-Neurotrophin 3 antibody (ab271274)

    Human Neurotrophin 3 was detected by sandwich ELISA (using 100 μl/well) using a concentration of 0.25-1.0 μg/ml of ab271274. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Neurotrophin 3 protein.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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