AB79962

Biotin Anti-Neutrophil Elastase antibody

Name: Anti-Neutrophil Elastase Biotin antibody (ab79962) IgG | Abcam
Brand: Abcam Limited
SKU: AB79962
Price: 525 USD
Availability: InStock

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(2 Publications)

Sheep Polyclonal Neutrophil Elastase antibody - conjugated to Biotin. Suitable for ELISA and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ELANE.

View Alternative Names

ELA2, ELANE, Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE

1 Images

ELISA - Biotin Anti-Neutrophil Elastase antibody (AB79962)

ELISA

Supplier Data

ELISA - Biotin Anti-Neutrophil Elastase antibody (AB79962)

Assay was performed using increasing concentrations of biotinylated purified human ELA2 protein. The antibody sensitivity is 30 pg/ml.

Key facts

Host species

Sheep

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

ELISA

applications

Immunogen

Native Full Length Protein corresponding to Human ELANE.

P08246

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.25% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neutrophil Elastase also known as neutrophil proteinase 3 has a molecular mass of approximately 30 kDa. It is a serine protease expressed mainly in azurophilic granules of neutrophils. This enzyme breaks down various proteins including elastin at sites of inflammation. Neutrophil Elastase releases during degranulation or through neutrophil extracellular traps (NETs) to combat pathogens.

Biological function summary

The target plays a significant role in neutrophil-mediated tissue remodeling and host defense. It leads to the breaking down of extracellular matrix components contributing to tissue remodeling and repair. Neutrophil Elastase is part of a complex interplay with other proteases like matrix metalloproteinases (MMPs). Its activity is regulated by endogenous inhibitors such as alpha-1 antitrypsin to prevent excessive tissue damage.

Pathways

This protease is important in inflammation and immune response pathways. It plays a role in the complement system by activating C5 a component of the complement cascade. It also acts in conjunction with other proteases such as cathepsin G to modulate immune and inflammatory responses. Neutrophil Elastase's role within these pathways is pivotal for the regulation of inflammation and destruction of pathogens.

Individuals with conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis often exhibit dysregulation or excessive activity of Neutrophil Elastase. This excessive activity leads to tissue damage and exacerbates disease progression. The enzyme also interacts with proteins like elastin and collagen in these diseases further contributing to pathological tissue remodeling and damage.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed : 15140022). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed : 36899106). Promotes blood coagulation (PubMed : 20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (PubMed : 25211214, PubMed : 9111081). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (PubMed : 20676107, PubMed : 25211214). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed : 10947984).

See full target information ELANE

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38928283

2024

The Effect of Targeted Hyperoxemia on Brain Immunohistochemistry after Long-Term, Resuscitated Porcine Acute Subdural Hematoma and Hemorrhagic Shock.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Münz,Thomas Datzmann,Andrea Hoffmann,Michael Gröger,René Mathieu,Simon Mayer,Fabian Zink,Holger Gässler,Eva-Maria Wolfschmitt,Melanie Hogg,Enrico Calzia,Pierre Asfar,Peter Radermacher,Thomas Kapapa,Tamara Merz

JACC. Basic to translational science 3:435-449 PubMed30175268

2018

Ticagrelor Reduces Thromboinflammatory Markers in Patients With Pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Travis R Sexton,Guoying Zhang,Tracy E Macaulay,Leigh A Callahan,Richard Charnigo,Olga A Vsevolozhskaya,Zhenyu Li,Susan Smyth

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Biotin Anti-Neutrophil Elastase antibody

ELISA - Biotin Anti-Neutrophil Elastase antibody (AB79962)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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