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AB271277

Biotin Anti-NGF antibody

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Rabbit Polyclonal NGF antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NGF.

View Alternative Names

NGFB, NGF, Beta-nerve growth factor, Beta-NGF

2 Images
ELISA - Biotin Anti-NGF antibody (AB271277)
  • ELISA

Unknown

ELISA - Biotin Anti-NGF antibody (AB271277)

Human NGF was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least XXXX of recombinant NGF protein.

Western blot - Biotin Anti-NGF antibody (AB271277)
  • WB

Supplier Data

Western blot - Biotin Anti-NGF antibody (AB271277)

Lane 1 : Marker.
Lane 2 : Recombinant hNGF at 250ng/lane.
Lane 3 : Recombinant hNGF at 125ng/lane.
Lane 4 : Recombinant hNGF at 62.5ng/lane.
Lane 5 : Recombinant hNGF at 31.25ng/lane.
Lane 6 : Recombinant hNGF at 15.625ng/lane.
Lane 7 : Recombinant hNGF at 7.8ng/lane.
Lane 8 : Recombinant hNGF at 3.9ng/lane.
Lane 9 : Recombinant hNGF at 1.95ng/lane.
Lane 10 : Recombinant hNGF at 0.975ng/lane.
Lane 11 : Recombinant hNGF at 0.4875ng/lane.
Lane 12 : Recombinant hNGF at 0.24ng/lane.

All lanes:

Western blot - Biotin Anti-NGF antibody (ab271277)

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

WB, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NGF.

P01138

Reactivity data

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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Purification technique
Affinity purification
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed : 14976160, PubMed : 20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed : 20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed : 20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).
See full target information NGF

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