Rabbit Polyclonal NGF antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NGF.
IgG
Rabbit
Biotin
Constituents: PBS, 0.1% BSA
Lyophilized
Polyclonal
ELISA | WB | |
---|---|---|
Recombinant fragment - Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info 0.25-1 µg/mL | Notes Human NGFwas detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant NGF protein. |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info 0.1-0.2 µg/mL | Notes When used in conjunction with compatible secondary reagents, the detection limit for recombinant human NGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed:14976160, PubMed:20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (PubMed:20978020) (Probable). The immature NGF precursor (proNGF) functions as ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).
Beta-nerve growth factor, Beta-NGF, NGFB, NGF
Rabbit Polyclonal NGF antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NGF.
Beta-nerve growth factor, Beta-NGF, NGFB, NGF
IgG
Rabbit
Biotin
Constituents: PBS, 0.1% BSA
Lyophilized
Polyclonal
Affinity purification
Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
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Lane 1: Marker.
Lane 2: Recombinant hNGF at 250ng/lane.
Lane 3: Recombinant hNGF at 125ng/lane.
Lane 4: Recombinant hNGF at 62.5ng/lane.
Lane 5: Recombinant hNGF at 31.25ng/lane.
Lane 6: Recombinant hNGF at 15.625ng/lane.
Lane 7: Recombinant hNGF at 7.8ng/lane.
Lane 8: Recombinant hNGF at 3.9ng/lane.
Lane 9: Recombinant hNGF at 1.95ng/lane.
Lane 10: Recombinant hNGF at 0.975ng/lane.
Lane 11: Recombinant hNGF at 0.4875ng/lane.
Lane 12: Recombinant hNGF at 0.24ng/lane.
All lanes: Western blot - Biotin Anti-NGF antibody (ab271277)
Predicted band size: 26 kDa
Human NGF was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least XXXX of recombinant NGF protein.
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