Rabbit Polyclonal NGF antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NGF.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human NGF. Database link P01138
Reactivity data
Select an application| ELISA | WB | |
|---|---|---|
| Recombinant fragment - Human | Tested | Tested |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 0.25-1 µg/mL | Notes Human NGFwas detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant NGF protein. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 0.1-0.2 µg/mL | Notes When used in conjunction with compatible secondary reagents, the detection limit for recombinant human NGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Target data
Function
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed:14976160, PubMed:20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed:20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).
Alternative names
NGFB, NGF, Beta-nerve growth factor, Beta-NGF
Recommended products
Rabbit Polyclonal NGF antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NGF.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human NGF. Database link P01138
- Purification technique
- Affinity purification
- Reconstitution
- Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Biotin Anti-NGF antibody (ab271277)
Lane 1: Marker.
Lane 2: Recombinant hNGF at 250ng/lane.
Lane 3: Recombinant hNGF at 125ng/lane.
Lane 4: Recombinant hNGF at 62.5ng/lane.
Lane 5: Recombinant hNGF at 31.25ng/lane.
Lane 6: Recombinant hNGF at 15.625ng/lane.
Lane 7: Recombinant hNGF at 7.8ng/lane.
Lane 8: Recombinant hNGF at 3.9ng/lane.
Lane 9: Recombinant hNGF at 1.95ng/lane.
Lane 10: Recombinant hNGF at 0.975ng/lane.
Lane 11: Recombinant hNGF at 0.4875ng/lane.
Lane 12: Recombinant hNGF at 0.24ng/lane.All lanes: Western blot - Biotin Anti-NGF antibody (ab271277)
Performed under non-reducing conditions.
Predicted band size: 26 kDa
ELISA - Biotin Anti-NGF antibody (ab271277)
Human NGF was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least XXXX of recombinant NGF protein.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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