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AB24496

Biotin Anti-Nitrotyrosine antibody [8C7.3]

5

(2 Reviews)

|

(2 Publications)

Mouse Monoclonal Nitrotyrosine antibody - conjugated to Biotin. Suitable for IP, ELISA, WB, IHC-P, IHC-Fr and reacts with Chemical samples. Cited in 2 publications.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8C7.3

Isotype

IgG2b

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

ELISA, IHC-P, WB, IP, IHC-Fr

applications

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Alternative Products

Primary Antibodies

AB27647

FITC Anti-Nitrotyrosine antibody

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Primary Antibodies

AB27648

HRP Anti-Nitrotyrosine antibody

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Primary Antibodies

AB27646

Biotin Anti-Nitrotyrosine antibody

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  • 0
Primary Antibodies

AB7048

Anti-Nitrotyrosine antibody [HM.11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nitrotyrosine antibody [HM.11] (AB7048)

  • 5
  • 13
Primary Antibodies

AB42789

Anti-Nitrotyrosine antibody

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  • 2

Complementary Products

Assay Kits

AB116691

3-Nitrotyrosine ELISA Kit

Biochemical assay - 3-Nitrotyrosine ELISA Kit (AB116691)

  • 4
  • 1
Primary Antibodies

AB42789

Anti-Nitrotyrosine antibody

  • 4
  • 2
Primary Antibodies

AB7048

Anti-Nitrotyrosine antibody [HM.11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nitrotyrosine antibody [HM.11] (AB7048)

  • 5
  • 13
Primary Antibodies

AB110282

Anti-3-Nitrotyrosine antibody [7A12AF6]

Flow Cytometry - Anti-3-Nitrotyrosine antibody [7A12AF6] (AB110282)

  • 0
  • 1
Primary Antibodies

AB61392

Anti-3-Nitrotyrosine antibody [39B6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-3-Nitrotyrosine antibody [39B6] (AB61392)

  • 4
  • 9
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nitrotyrosine also known as 3-nitrotyrosine is a marker for nitrosative stress and oxidative damage. It comes from the nitration of tyrosine an amino acid primarily through the action of reactive nitrogen species. Nitrotyrosine can be found in proteins and its molecular mass varies depending on the protein context. It is present across various tissues and fluid samples within the body particularly where high oxidative stress occurs.
Biological function summary

Nitrotyrosine acts as an indicator of cellular damage and protein modification due to stress conditions. When proteins have nitrotyrosine residues their function might change which can lead to cellular signaling disruption. Nitrotyrosine is not usually part of a specific complex but it affects proteins and enzymes by altering their activity and stability contributing to larger stress response mechanisms.

Pathways

Proteins modified by nitrotyrosine are involved in cellular pathways related to oxidative stress and inflammation. Particularly pathways like the nitric oxide synthase pathway and the inflammatory response pathway include nitrotyrosine-modified proteins. It is related to proteins such as nitric oxide synthase (NOS) and superoxide dismutase (SOD) important in managing oxidative balance and stress responses in cells.

Nitrotyrosine is significantly linked to diseases related to oxidative stress such as cardiovascular disease and neurodegenerative disorders. These conditions often have increased levels of nitrotyrosine indicating heightened oxidative damage. Through these diseases nitrotyrosine connects with proteins like amyloid-beta in neurodegenerative disorders and endothelial nitric oxide synthase (eNOS) in cardiovascular diseases reflecting the impact of oxidative stress on these proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Nitrotyrosine
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Analytical and bioanalytical chemistry 410:1389-1396 PubMed29214534

2017

Development of a sandwich ELISA with potential for selective quantification of human lactoferrin protein nitrated through disease or environmental exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Amani Y Alhalwani,John E Repine,Michelle K Knowles,J Alex Huffman

The EMBO journal 32:2603-16 PubMed24013120

2013

The NAD-dependent deacetylase sirtuin 2 is a suppressor of microglial activation and brain inflammation.

Applications

IHC-P

Species

Mouse

Teresa Faria Pais,Éva M Szegő,Oldriska Marques,Leonor Miller-Fleming,Pedro Antas,Patrícia Guerreiro,Rita Machado de Oliveira,Burcu Kasapoglu,Tiago Fleming Outeiro
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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