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AB183434

Biotin Anti-NMDAR1 antibody [N308/48]

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(1 Publication)

Mouse Monoclonal NMDAR1 antibody - conjugated to Biotin. Suitable for WB and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Rat Grin1 aa 1-400.

View Alternative Names

NMDAR1, GRIN1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, hNR1

1 Images
Western blot - Biotin Anti-NMDAR1 antibody [N308/48] (AB183434)
  • WB

Supplier Data

Western blot - Biotin Anti-NMDAR1 antibody [N308/48] (AB183434)

Lane 1:

Western blot - Biotin Anti-NMDAR1 antibody [N308/48] (ab183434) at 1/1000 dilution

Lane 1:

Western blot - HRP Anti-NMDAR1 antibody [N308/48] - N-terminal (<a href='/en-us/products/primary-antibodies/hrp-nmdar1-antibody-n308-48-n-terminal-ab183433'>ab183433</a>) at 1/1000 dilution

All lanes:

rat brain membrane tissue lysate

Predicted band size: 105 kDa

false

  • Unconjugated

    Anti-NMDAR1 antibody [N308/48] - Neuronal Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N308/48

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Rat Grin1 aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

P35439

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>1 μg/ml of ab183434 is sufficient for detection of NMDAR1 in 20μg of rat brain membrane lysate.</p>" } } }
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Product details

The clone number has been updated from S308-48 to N308/48, both clone numbers name the same antibody clone.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR1 also known as GluN1 is a subunit of the NMDA receptor a type of ion channel protein found in the brain. This receptor weighs around 120 kDa and plays an important role in synaptic transmission and plasticity. NMDAR1 operates as part of a receptor complex that allows for calcium influx when activated. It shows high expression throughout the central nervous system primarily in neurons across the cortex and hippocampus. The receptor's function is important to certain calcium-dependent processes in the brain.
Biological function summary

NMDA receptors including the NMDAR1 subunit serve essential functions in synaptic plasticity and memory processes. As part of the receptor complex NMDAR1 contributes to the control of synaptic strength. It requires co-activation by glutamate and glycine or D-serine linking its function to excitatory neurotransmission. The receptor's capacity to modulate synaptic connections forms the basis of learning and long-term memory.

Pathways

NMDAR1 plays a critical role in the glutamatergic pathway influencing synaptic plasticity and memory function. It interacts with other proteins like PSD-95 within the postsynaptic density. NMDAR1 is also involved in the calcium signaling pathway due to its ability to facilitate calcium entry into neurons upon synaptic activity. This positions the receptor as an important modulator in pathways that control neuronal communication and plasticity.

NMDA receptor dysfunction including the NMDAR1 subunit links to neurodegenerative diseases such as Alzheimer's. The alterations in NMDAR1 function can lead to excitotoxicity contributing to neuronal damage. NMDAR1 also associates with schizophrenia where altered glutamatergic transmission has been observed. Here abnormalities in proteins like the synaptic scaffolding protein PSD-95 have also been noted to relate to NMDAR1 dysfunction in the pathophysiology of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 21376300, PubMed : 26875626, PubMed : 26919761, PubMed : 28126851, PubMed : 28228639, PubMed : 36959261, PubMed : 7679115, PubMed : 7681588, PubMed : 7685113). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP) (PubMed : 26875626). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 21376300, PubMed : 26875626, PubMed : 26919761, PubMed : 27164704, PubMed : 28095420, PubMed : 28105280, PubMed : 28126851, PubMed : 28228639, PubMed : 36959261, PubMed : 38538865, PubMed : 7679115, PubMed : 7681588, PubMed : 7685113). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 26875626, PubMed : 26919761, PubMed : 36309015, PubMed : 38598639).
See full target information GRIN1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genetics and molecular research : GMR 15: PubMed27525866

2016

KCNQ1 A340E impairs electrolyte homeostasis independently of the renin-angiotensin-aldosterone system in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Q Pan,Y Sang,C Sun,G Li,Y Wang
View all publications
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