Rat Monoclonal PDGFR beta antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Protein within Mouse Pdgfrb.
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![Flow Cytometry - Biotin Anti-PDGFR beta antibody [APB5] (AB93533), expandable thumbnail](https://content.abcam.com/products/images/biotin-pdgfr-beta-antibody-apb5-ab93533--flow-cytometry-img18254.gif "Flow Cytometry - Biotin Anti-PDGFR beta antibody [APB5] (AB93533)")
Publications
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- Annals of the New York Academy of Sciences 947:303-52002Study on PDGF receptor beta pathway in glomerular formation in neonate mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesH Sano,M Yokode,N Takakura,G Takemura,T Doi,H Kataoka,T Sudo,S Nishikawa,H Fujiwara,S I Nishikawa,T KitaPubMed 11795278
- Circulation 103:2955-602001Functional blockade of platelet-derived growth factor receptor-beta but not of receptor-alpha prevents vascular smooth muscle cell accumulation in fibrous cap lesions in apolipoprotein E-deficient mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesH Sano,T Sudo,M Yokode,T Murayama,H Kataoka,N Takakura,S Nishikawa,S I Nishikawa,T KitaPubMed 11413086
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 45:883-931997PDGFR alpha expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal anti-mouse PDGFR alpha antibody APA5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesN Takakura,H Yoshida,Y Ogura,H Kataoka,S Nishikawa,S NishikawaPubMed 9199674
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Conjugation
- Biotin
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Protein within Mouse Pdgfrb. The exact immunogen used to generate this antibody is proprietary information. Database link P05622
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Mouse | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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6 products for Alternative Product
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM (By similarity). Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
Alternative names
CD140b, Pdgfr, Pdgfr1, Pdgfrb, Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1
Recommended products
Rat Monoclonal PDGFR beta antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Protein within Mouse Pdgfrb.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Conjugation
- Biotin
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Protein within Mouse Pdgfrb. The exact immunogen used to generate this antibody is proprietary information. Database link P05622
- Clone number
- APB5
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.
- Biological function summary
The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.
- Pathways
PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.
- Associated diseases and disorders
PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.
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Tested
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
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Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Flow Cytometry - Biotin Anti-PDGFR beta antibody [APB5] (ab93533)
ab93533 at 0.5μg dilution staining PDGFR beta in NIH3T3 cells by Flow Cytometry (shown in blue) followed by Streptavidin-PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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