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AB222255

Biotin Anti-Phosphotyrosine antibody [EPR16871]

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(1 Publication)

Rabbit Recombinant Monoclonal Phosphotyrosine antibody - conjugated to Biotin. Suitable for ELISA and reacts with Modified Amino Acid samples. Cited in 1 publication.

View Alternative Names

L-Phosphotyrosine, L-Tyrosine-O-phosphate, O-Phospho-L-tyrosine, O-Phosphotyrosine, Phospho-L-tyrosine, Phosphonotyrosine

1 Images
ELISA - Biotin Anti-Phosphotyrosine antibody [EPR16871] (AB222255)
  • ELISA

Lab

ELISA - Biotin Anti-Phosphotyrosine antibody [EPR16871] (AB222255)

Serially diluted ab222255 was bound to immobilised phospho- or control peptides (STAT1 (phospho S727), STAT1 control, STAT5 (phospho T694), STAT5 control); 1 microgram per mL).

The antibody was detected by HRP-labelled streptavidin (ab7403; diluted 10000 times) and signal was developed by TMB substrate.

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Phosphotyrosine antibody [EPR16871]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Phosphotyrosine antibody [EPR16871]

  • Unconjugated

    Anti-Phosphotyrosine antibody [EPR16871]

  • Carrier free

    Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16871

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "2-10 µg/mL", "ELISA-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.
Biological function summary

Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.

Pathways

The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.

The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Phosphotyrosine
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:647763 PubMed34055778

2021

EPC-Derived Exosomal miR-1246 and miR-1290 Regulate Phenotypic Changes of Fibroblasts to Endothelial Cells to Exert Protective Effects on Myocardial Infarction by Targeting ELF5 and SP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yulang Huang,Lifang Chen,Zongming Feng,Weixin Chen,Shaodi Yan,Rongfeng Yang,Jian Xiao,Jiajia Gao,Debao Zhang,Xiao Ke
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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