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AB53449

Biotin Anti-Polyethylene glycol antibody [PEG-B-47b]

5

(1 Review)

|

(15 Publications)

Anti-Polyethylene glycol antibody [PEG-B-47b] - Biotin conjugated (ab53449) is a rabbit recombinant monoclonal antibody detecting Polyethylene glycol in Western Blot, IHC-P, ELISA.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
- Trusted since 2007

View Alternative Names

PEG

6 Images
ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)
  • ELISA

Unknown

ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Direct ELISA antigen dose-response curve using purified ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.

Biotin conjugate (ab53449) available for this clone.

ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)
  • ELISA

Unknown

ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

ELISA assay using 5 μg/mL Anti-AFP RabMAb as the capture antibody and 0.5 μg/mL Anti-PEG-47-Biotin RabMAb (unpurified ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.

Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)
  • sELISA

Unknown

Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Sandwich ELISA antigen dose-response curve using purified ab53449.

Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).

Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).

Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).

ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)
  • ELISA

Unknown

ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified ab53449).

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)
  • WB

Lab

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/50000 dilution

All lanes:

BSA-PEG5K at 0.02 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 2 kDa

Observed band size: 280 kDa

false

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)
  • WB

Unknown

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Lane 1:

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/5000 dilution

Lane 2:

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/10000 dilution

Lane 3:

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/20000 dilution

All lanes:

BSA-PEG

Predicted band size: 2 kDa

false

  • Carrier free

    Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

PEG-B-47b

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

WB, sELISA, IHC-P, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab53449 does not cross react with Tween20.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1 µg/mL", "ELISA-species-notes": "<p>Coating plates for ELISA: 0 to 500 ng/ml.</p><p>For unpurified use at 0.5μg/ml, coating plates for ELISA: 5 to 10 ug/ml.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000", "WB-species-notes": "<p><strong>For unpurified use at 1/5000 - 1/20000.</strong></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "<p><strong>For unpurified use at 1/50 - 1/100. Images of positive IHC with this monoclonal antibody, clone PEG-B-47b, are on the <a href='/en-us/products/primary-antibodies/polyethylene-glycol-antibody-peg-b-47-ab51257'>ab51257</a> datasheet.</strong></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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AB215546

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Polyethylene glycol
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of colloid and interface science 610:766-774 PubMed34848062

2021

Screening of the binding affinity of serum proteins to lipid nanoparticles in a cell free environment.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Sebastiani,Marianna Yanez Arteta,Lennart Lindfors,Marité Cárdenas

ACS nano 15:6709-6722 PubMed33754708

2021

Apolipoprotein E Binding Drives Structural and Compositional Rearrangement of mRNA-Containing Lipid Nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Sebastiani,Marianna Yanez Arteta,Michael Lerche,Lionel Porcar,Christian Lang,Ryan A Bragg,Charles S Elmore,Venkata R Krishnamurthy,Robert A Russell,Tamim Darwish,Harald Pichler,Sarah Waldie,Martine Moulin,Michael Haertlein,V Trevor Forsyth,Lennart Lindfors,Marité Cárdenas

Transplantation 104:744-753 PubMed31929419

2020

Liposomal Delivery Improves the Efficacy of Prednisolone to Attenuate Renal Inflammation in a Mouse Model of Acute Renal Allograft Rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Carla M A van Alem,Martina Schmidbauer,Song Rong,Katja Derlin,Jessica Schmitz,Jan H Bräsen,Anja Thorenz,Rongjun Chen,Jurjen M Ruben,Elizabeth M Winter,Maaike Schilperoort,Sander Kooijman,Reshma A Lalai,Josbert M Metselaar,Christian Klemann,Martin Meier,Cees van Kooten,Faikah Gueler,Joris I Rotmans

Stem cell reports 14:91-104 PubMed31839542

2019

Conformal Coating of Stem Cell-Derived Islets for β Cell Replacement in Type 1 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron A Stock,Vita Manzoli,Teresa De Toni,Maria M Abreu,Yeh-Chuin Poh,Lillian Ye,Adam Roose,Felicia W Pagliuca,Chris Thanos,Camillo Ricordi,Alice A Tomei

Acta biomaterialia 97:461-473 PubMed31344512

2019

Computer-assisted engineering of programmed drug releasing multilayer nanomedicine via indomethacin-mediated ternary complex for therapy against a multidrug resistant tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Che,Zhirui Liu,Dong Wang,Chunmei Xu,Chengyuan Zhang,Jin Meng,Jun Zheng,Hongfeng Yuan,Guanren Zhao,Xing Zhou

Nanomedicine : nanotechnology, biology, and medici 11:1039-46 PubMed25791806

2015

Prednisolone-containing liposomes accumulate in human atherosclerotic macrophages upon intravenous administration.

Applications

Unspecified application

Species

Unspecified reactive species

Fleur M van der Valk,Diederik F van Wijk,Mark E Lobatto,Hein J Verberne,Gert Storm,Martine C M Willems,Dink A Legemate,Aart J Nederveen,Claudia Calcagno,Venkatesh Mani,Sarayu Ramachandran,Maarten P M Paridaans,Maarten J Otten,Geesje M Dallinga-Thie,Zahi A Fayad,Max Nieuwdorp,Dominik M Schulte,Josbert M Metselaar,Willem J M Mulder,Erik S Stroes

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 15:88-100 PubMed25488654

2014

FR104, an antagonist anti-CD28 monovalent fab' antibody, prevents alloimmunization and allows calcineurin inhibitor minimization in nonhuman primate renal allograft.

Applications

Unspecified application

Species

Unspecified reactive species

N Poirier,N Dilek,C Mary,S Ville,F Coulon,J Branchereau,X Tillou,V Charpy,S Pengam,V Nerriere-Daguin,J Hervouet,D Minault,S Le Bas-Bernardet,K Renaudin,B Vanhove,G Blancho

Journal of immunology (Baltimore, Md. : 1950) 191:4599-610 PubMed24081989

2013

A monovalent anti-human CD28 domain antibody antagonist: preclinical efficacy and safety.

Applications

ELISA

Species

Unspecified reactive species

Suzanne J Suchard,Patricia M Davis,Selena Kansal,Dawn K Stetsko,Ruth Brosius,James Tamura,Lumelle Schneeweis,James Bryson,Theodora Salcedo,Haiqing Wang,Zheng Yang,Catherine A Fleener,Olga Ignatovich,Christopher Plummer,Steven Grant,Steven G Nadler

Growth hormone & IGF research : official journal o 23:114-9 PubMed23651793

2013

Pegvisomant bioavailability of single 30 mg/mL subcutaneous injection compared to two 15 mg/mL subcutaneous injections: a pharmacokinetic, safety and tolerability study.

Applications

ELISA

Species

Unspecified reactive species

Juif Jen,Robert R LaBadie,Yali Liang,Penelope H Crownover,Xiang Gao,Juliana H Hey-Hadavi

Inflammatory bowel diseases 19:1224-31 PubMed23619715

2013

The cytotoxic effects of certolizumab pegol and golimumab mediated by transmembrane tumor necrosis factor α.

Applications

Unspecified application

Species

Unspecified reactive species

Naoyasu Ueda,Hiroshi Tsukamoto,Hiroki Mitoma,Masahiro Ayano,Atsushi Tanaka,Shun-ichiro Ohta,Yasushi Inoue,Yojiro Arinobu,Hiroaki Niiro,Koichi Akashi,Takahiko Horiuchi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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