Anti-Polyethylene glycol antibody [PEG-B-47b] is a rabbit recombinant monoclonal Biotin conjugated antibody that is used to detect Polyethylene glycol in ELISA, IHC-P, Western blot, sELISA.
- Detects PEG molecules from PEG750 to PEG 40K, covering a wide molecular range
- Recognizes both linear and branched PEG structures for versatile applications
- Cited in over 10 publications
Images
![ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449), expandable thumbnail](https://content.abcam.com/products/images/biotin-polyethylene-glycol-antibody-peg-b-47b-ab53449--elisa-img116420.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)")
![Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449), expandable thumbnail](https://content.abcam.com/products/images/biotin-polyethylene-glycol-antibody-peg-b-47b-ab53449--sandwich-elisa-img121759.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)")
![Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449), expandable thumbnail](https://content.abcam.com/products/images/biotin-polyethylene-glycol-antibody-peg-b-47b-ab53449--western-blot-img5312.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)")
![ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449), expandable thumbnail](https://content.abcam.com/products/images/biotin-polyethylene-glycol-antibody-peg-b-47b-ab53449--elisa-img101631.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)")
![Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449), expandable thumbnail](https://content.abcam.com/products/images/biotin-polyethylene-glycol-antibody-peg-b-47b-ab53449--western-blot-img106462.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)")
Publications
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- Journal of colloid and interface science 610:766-7742021Screening of the binding affinity of serum proteins to lipid nanoparticles in a cell free environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFederica Sebastiani,Marianna Yanez Arteta,Lennart Lindfors,Marité CárdenasPubMed 34848062
- ACS nano 15:6709-67222021Apolipoprotein E Binding Drives Structural and Compositional Rearrangement of mRNA-Containing Lipid Nanoparticles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFederica Sebastiani,Marianna Yanez Arteta,Michael Lerche,Lionel Porcar,Christian Lang,Ryan A Bragg,Charles S Elmore,Venkata R Krishnamurthy,Robert A Russell,Tamim Darwish,Harald Pichler,Sarah Waldie,Martine Moulin,Michael Haertlein,V Trevor Forsyth,Lennart Lindfors,Marité CárdenasPubMed 33754708
- Transplantation 104:744-7532020Liposomal Delivery Improves the Efficacy of Prednisolone to Attenuate Renal Inflammation in a Mouse Model of Acute Renal Allograft Rejection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarla M A van Alem,Martina Schmidbauer,Song Rong,Katja Derlin,Jessica Schmitz,Jan H Bräsen,Anja Thorenz,Rongjun Chen,Jurjen M Ruben,Elizabeth M Winter,Maaike Schilperoort,Sander Kooijman,Reshma A Lalai,Josbert M Metselaar,Christian Klemann,Martin Meier,Cees van Kooten,Faikah Gueler,Joris I RotmansPubMed 31929419
- Stem cell reports 14:91-1042019Conformal Coating of Stem Cell-Derived Islets for β Cell Replacement in Type 1 Diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAaron A Stock,Vita Manzoli,Teresa De Toni,Maria M Abreu,Yeh-Chuin Poh,Lillian Ye,Adam Roose,Felicia W Pagliuca,Chris Thanos,Camillo Ricordi,Alice A TomeiPubMed 31839542
- Acta biomaterialia 97:461-4732019Computer-assisted engineering of programmed drug releasing multilayer nanomedicine via indomethacin-mediated ternary complex for therapy against a multidrug resistant tumor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Che et. al.PubMed 31344512
- Nanomedicine : nanotechnology, biology, and medici 11:1039-462015Prednisolone-containing liposomes accumulate in human atherosclerotic macrophages upon intravenous administration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFleur M van der Valk et. al.PubMed 25791806
- American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 15:88-1002014FR104, an antagonist anti-CD28 monovalent fab' antibody, prevents alloimmunization and allows calcineurin inhibitor minimization in nonhuman primate renal allograft.Applications:Unspecified applicationReactive species:Unspecified reactive speciesN Poirier,N Dilek,C Mary,S Ville,F Coulon,J Branchereau,X Tillou,V Charpy,S Pengam,V Nerriere-Daguin,J Hervouet,D Minault,S Le Bas-Bernardet,K Renaudin,B Vanhove,G BlanchoPubMed 25488654
- Journal of immunology (Baltimore, Md. : 1950) 191:4599-6102013A monovalent anti-human CD28 domain antibody antagonist: preclinical efficacy and safety.Applications:ELISAReactive species:Unspecified reactive speciesSuzanne J Suchard et. al.PubMed 24081989
- Growth hormone & IGF research : official journal o 23:114-92013Pegvisomant bioavailability of single 30 mg/mL subcutaneous injection compared to two 15 mg/mL subcutaneous injections: a pharmacokinetic, safety and tolerability study.
- Inflammatory bowel diseases 19:1224-312013The cytotoxic effects of certolizumab pegol and golimumab mediated by transmembrane tumor necrosis factor α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaoyasu Ueda,Hiroshi Tsukamoto,Hiroki Mitoma,Masahiro Ayano,Atsushi Tanaka,Shun-ichiro Ohta,Yasushi Inoue,Yojiro Arinobu,Hiroaki Niiro,Koichi Akashi,Takahiko HoriuchiPubMed 23619715
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ELISA | WB | sELISA | IHC-P | |
|---|---|---|---|---|
| Chemical | Tested | Tested | Tested | Expected |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1 µg/mL | Notes Coating plates for ELISA: 0 to 500 ng/ml. For unpurified use at 0.5μg/ml, coating plates for ELISA: 5 to 10 ug/ml. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/50000 | Notes For unpurified use at 1/5000 - 1/20000. |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/250 | Notes For unpurified use at 1/50 - 1/100. Images of positive IHC with this monoclonal antibody, clone PEG-B-47b, are on the ab51257 datasheet. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
3 products for Alternative Version
5 products for Alternative Product
Alternative names
PEG
Recommended products
Anti-Polyethylene glycol antibody [PEG-B-47b] is a rabbit recombinant monoclonal Biotin conjugated antibody that is used to detect Polyethylene glycol in ELISA, IHC-P, Western blot, sELISA.
- Detects PEG molecules from PEG750 to PEG 40K, covering a wide molecular range
- Recognizes both linear and branched PEG structures for versatile applications
- Cited in over 10 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- PEG-B-47b
- Purification technique
- Affinity purification Protein A
- Specificity
ab53449 does not cross react with Tween20.
- Dissociation constant
- 2.41 x 10-9 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ELISA, IHC-P, WB, sELISA.
Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) has been cited over 15 times in peer reviewed journals and is trusted by the scientific community.
Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) is sold in a 100 ug selling size.
Conjugation-ready, carrier free format available for antibody clone (PEG-B-47b) - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free ab271853.
The smallest PEG molecule which our Polyethylene glycol antibody (ab53449) can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab53449 detects both linear and branched PEG based on our in-house ELISA testing.
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody [PEG-B-47b] reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody.
If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449), expandable thumbnail](https://content.abcam.com/products/images/biotin-polyethylene-glycol-antibody-peg-b-47b-ab53449--elisa-img116420.jpg "ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)")
ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
Direct ELISA antigen dose-response curve using purified ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.
Biotin conjugate (ab53449) available for this clone.
![Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449), expandable thumbnail](https://content.abcam.com/products/images/biotin-polyethylene-glycol-antibody-peg-b-47b-ab53449--sandwich-elisa-img121759.jpg "Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)")
Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
Sandwich ELISA antigen dose-response curve using purified ab53449.
Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).
Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).
Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).
![Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449), expandable thumbnail](https://content.abcam.com/products/images/biotin-polyethylene-glycol-antibody-peg-b-47b-ab53449--western-blot-img5312.jpg "Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)")
Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/50000 dilution
All lanes: BSA-PEG5K at 0.02 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 2 kDa
Observed band size: 280 kDa
![ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449), expandable thumbnail](https://content.abcam.com/products/images/biotin-polyethylene-glycol-antibody-peg-b-47b-ab53449--elisa-img101631.jpg "ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)")
ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
ELISA assay using 5 μg/mL Anti-AFP RabMAb as the capture antibody and 0.5 μg/mL Anti-PEG-47-Biotin RabMAb (unpurified ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.
![Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449), expandable thumbnail](https://content.abcam.com/products/images/biotin-polyethylene-glycol-antibody-peg-b-47b-ab53449--western-blot-img106462.jpg "Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)")
Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
Lane 1: Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/5000 dilution
Lane 2: Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/10000 dilution
Lane 3: Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/20000 dilution
All lanes: BSA-PEG
Predicted band size: 2 kDa
![ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449), expandable thumbnail](https://content.abcam.com/products/images/biotin-polyethylene-glycol-antibody-peg-b-47b-ab53449--elisa-img105522.jpg "ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)")
ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified ab53449).
![ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449), expandable thumbnail](https://content.abcam.com/products/images/biotin-polyethylene-glycol-antibody-peg-b-47b-ab53449--elisa-img105522.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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