Rat Monoclonal RANKL antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tnfsf11.
Images
![Flow Cytometry - Biotin Anti-RANKL antibody [IK22/5] (AB95720), expandable thumbnail](https://content.abcam.com/products/images/biotin-rankl-antibody-ik22-5-ab95720--flow-cytometry-img53670.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Biotin Anti-RANKL antibody [IK22/5] (AB95720)")
Publications
Filter by
- Journal of immunology (Baltimore, Md. : 1950) 182:5439-452009LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.Applications:IFReactive species:Unspecified reactive speciesMark F Vondenhoff,Mascha Greuter,Gera Goverse,Dirk Elewaut,Pieter Dewint,Carl F Ware,Kerim Hoorweg,Georg Kraal,Reina E MebiusPubMed 19380791
- Biochemical and biophysical research communication 347:124-322006Amelioration of bone loss in collagen-induced arthritis by neutralizing anti-RANKL monoclonal antibody.Applications:Flow CytReactive species:Unspecified reactive speciesSeiji Kamijo et. al.PubMed 16815304
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Conjugation
- Biotin
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Mouse Tnfsf11. Database link O35235
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Mouse | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.12500-0.25000 µg for 105-108 Cells | Notes A final volume of 100 μL is recommended for staining the cell sample. ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
5 products for Alternative Product
1 product for Alternative Version
Target data
Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor (PubMed:22437732). Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (By similarity). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts (PubMed:18586671, PubMed:24039232, PubMed:27336669). During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (PubMed:23395171, PubMed:26644563).
Alternative names
CD254, Opgl, Rankl, Trance, Tnfsf11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, OPGL, RANKL, TRANCE
Recommended products
Rat Monoclonal RANKL antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tnfsf11.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Conjugation
- Biotin
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Mouse Tnfsf11. Database link O35235
- Clone number
- IK22/5
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - Biotin Anti-RANKL antibody [IK22/5] (ab95720), expandable thumbnail](https://content.abcam.com/products/images/biotin-rankl-antibody-ik22-5-ab95720--flow-cytometry-img53670.jpg "Flow Cytometry - Biotin Anti-RANKL antibody [IK22/5] (ab95720)")
Flow Cytometry - Biotin Anti-RANKL antibody [IK22/5] (ab95720)
Staining of 3 day unstimulated (left) and 3 day ConA activated (right) BALB/c splenocytes with 0.125 μg of Biotin Rat IgG2a Isotype Control (open histogram) or 0.125 μg of ab95720 (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com